biostudies-literatureGibbs NMNCRR NIH HHSNational Aeronautics and Space AdministrationUniversity of Wisconsin-MadisonNational Institutes of HealthNIGMS NIH HHS1283-1298https://www.ebi.ac.uk/biostudies/studies/S-EPMC8518694biostudies-literatureUnknown107(5)Cadaverine, a polyamine, has been linked to modification of root growth architecture and response to environmental stresses in plants. However, the molecular mechanisms that govern the regulation of root growth by cadaverine are largely unexplored. Here we conducted a forward genetic screen and isolated a mutation, cadaverine hypersensitive 3 (cdh3), which resulted in increased root-growth sensitivity to cadaverine, but not other polyamines. This mutation affects the BIO3-BIO1 biotin biosynthesis gene. Exogenous supply of biotin and a pathway intermediate downstream of BIO1, 7,8-diaminopelargonic acid, suppressed this cadaverine sensitivity phenotype. An in vitro enzyme assay showed cadaverine inhibits the BIO3-BIO1 activity. Furthermore, cadaverine-treated seedlings displayed reduced biotThe Plant journal : for cell and molecular biologyCadaverine regulates biotin synthesis to modulate primary root growth in Arabidopsis.PMC8518694P20 GM103418S10RR13790#80NSSC19K148380NSSC19K1483#MSN226688S10 RR013790Su SHAlban CMaeda HAMasson PHGibbs NMMann SLopez-Nieves SfalseCadaverine regulates biotin synthesis to modulate primary root growth in Arabidopsis.Cadaverine, a polyamine, has been linked to modification of root growth architecture and response to environmental stresses in plants. However, the molecular mechanisms that govern the regulation of root growth by cadaverine are largely unexplored. Here we conducted a forward genetic screen and isolated a mutation, cadaverine hypersensitive 3 (cdh3), which resulted in increased root-growth sensitivity to cadaverine, but not other polyamines. This mutation affects the BIO3-BIO1 biotin biosynthesis gene. Exogenous supply of biotin and a pathway intermediate downstream of BIO1, 7,8-diaminopelargonic acid, suppressed this cadaverine sensitivity phenotype. An in vitro enzyme assay showed cadaverine inhibits the BIO3-BIO1 activity. Furthermore, cadaverine-treated seedlings displayed reduced biot2021-01-01T00:00:00Z2021 Sep2026-03-27T16:15:21.978Z2025-02-19T00:18:30.122ZS-EPMC85186943425067010.1111/tpj.15417