biostudies-literatureUnknown8Gabbia DUniversità degli Studi di PadovaLiver fibrosis, which is the outcome of wound-healing response to chronic liver damage, represents an unmet clinical need. This study evaluated the anti-fibrotic and anti-inflammatory effects of the polyphenol oleocanthal (OC) extracted from extra virgin olive oil (EVOO) by an <i>in vitro/in vivo</i> approach. The hepatic cell lines LX2 and HepG2 were used as <i>in vitro</i> models. The mRNA expression of pro-fibrogenic markers, namely alpha-smooth muscle actin (α-SMA), collagen type I alpha 1 chain (COL1A1), a panel of metalloproteinases (MMP1, MMP2, MMP3, MMP7, MMP9) and vascular endothelial growth factor A (VEGFA) as well as the pro-oxidant genes NADPH oxidases (NOXs) 1 and 4 were evaluated in TGF-β activated LX2 cells by qRT-PCR. α-SMA and COL1A1 protein expression was assessed by immuFrontiers in nutrition715183https://www.ebi.ac.uk/biostudies/studies/S-EPMC8521071biostudies-literatureThe Extra Virgin Olive Oil Polyphenol Oleocanthal Exerts Antifibrotic Effects in the Liver.PMC8521071Esposito Salsano JRusso FPPolini BCarpi SColognesi MNieri PCarrara MSarcognato SDigiacomo MGuido MManera CScaffidi MMacchia MCannella LGabbia DDe Martin SfalseThe Extra Virgin Olive Oil Polyphenol Oleocanthal Exerts Antifibrotic Effects in the Liver.Liver fibrosis, which is the outcome of wound-healing response to chronic liver damage, represents an unmet clinical need. This study evaluated the anti-fibrotic and anti-inflammatory effects of the polyphenol oleocanthal (OC) extracted from extra virgin olive oil (EVOO) by an <i>in vitro/in vivo</i> approach. The hepatic cell lines LX2 and HepG2 were used as <i>in vitro</i> models. The mRNA expression of pro-fibrogenic markers, namely alpha-smooth muscle actin (α-SMA), collagen type I alpha 1 chain (COL1A1), a panel of metalloproteinases (MMP1, MMP2, MMP3, MMP7, MMP9) and vascular endothelial growth factor A (VEGFA) as well as the pro-oxidant genes NADPH oxidases (NOXs) 1 and 4 were evaluated in TGF-β activated LX2 cells by qRT-PCR. α-SMA and COL1A1 protein expression was assessed by immu2021-01-01T00:00:00Z20212025-04-21T14:48:42.415Z2025-04-21T14:48:42.415ZS-EPMC85210713467163010.3389/fnut.2021.715183