biostudies-literatureUnknown11Huang C<h4>Background</h4>The usefulness of metagenomic next-generation sequencing (mNGS) in identifying pathogens is being investigated. We aimed to compare the power of microbial identification between mNGS and various methods in patients with acute respiratory failure.<h4>Methods</h4>We reviewed 130 patients with respiratory failure, and 184 specimens including blood, bronchoalveolar lavage fluid (BALF), sputum, pleural effusion, ascitic fluid, and urine were tested by mNGS and conventional methods (culture, PCR). We also enrolled 13 patients to evaluate the power of mNGS and pathogen targets NGS (ptNGS) in microbial identifications. Clinical features and microbes detected were analyzed.<h4>Results</h4>mNGS outperformed the conventional method in the positive detection rate of <i>MycobacteriumFrontiers in cellular and infection microbiology738074https://www.ebi.ac.uk/biostudies/studies/S-EPMC8522648biostudies-literatureA Microbial World: Could Metagenomic Next-Generation Sequencing Be Involved in Acute Respiratory Failure?PMC8522648Huang CDing YSummah HDFeng YMa XDu WShi GZhu HChen HZhang SfalseA Microbial World: Could Metagenomic Next-Generation Sequencing Be Involved in Acute Respiratory Failure?<h4>Background</h4>The usefulness of metagenomic next-generation sequencing (mNGS) in identifying pathogens is being investigated. We aimed to compare the power of microbial identification between mNGS and various methods in patients with acute respiratory failure.<h4>Methods</h4>We reviewed 130 patients with respiratory failure, and 184 specimens including blood, bronchoalveolar lavage fluid (BALF), sputum, pleural effusion, ascitic fluid, and urine were tested by mNGS and conventional methods (culture, PCR). We also enrolled 13 patients to evaluate the power of mNGS and pathogen targets NGS (ptNGS) in microbial identifications. Clinical features and microbes detected were analyzed.<h4>Results</h4>mNGS outperformed the conventional method in the positive detection rate of <i>Mycobacterium2021-01-01T00:00:00Z20212026-05-08T19:48:44.778Z2024-11-20T02:26:32.841ZS-EPMC85226483467156910.3389/fcimb.2021.738074