{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Voss G"],"funding":["Vetenskapsrådet","Kungliga Fysiografiska Sällskapet i Lund","Prostatacancerfonden","Cancerfonden"],"pagination":["1412-1424"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8522694"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(11)"],"pubmed_abstract":["Even though microRNAs have been viewed as promising biomarkers for years, their clinical implementation is still lagging far behind. This is in part due to the lack of RT-qPCR technologies that can differentiate between microRNA isoforms. For example, A-to-I editing of microRNAs through adenosine deaminase acting on RNA (ADAR) enzymes can affect their expression levels and functional roles, but editing isoform-specific assays are not commercially available. Here, we describe RT-qPCR assays that are specific for editing isoforms, using microRNA-379 (miR-379) as a model. The assays are based on two-tailed RT-qPCR, and we show them to be compatible both with SYBR Green and hydrolysis-based chemistries, as well as with both qPCR and digital PCR. The assays could readily detect different miR-37"],"journal":["RNA (New York, N.Y.)"],"pubmed_title":["Quantification of microRNA editing using two-tailed RT-qPCR for improved biomarker discovery."],"pmcid":["PMC8522694"],"funding_grant_id":["CAN 2017/559","VR-MH 2018-03125","40526"],"pubmed_authors":["Voss G","Edsjo A","Ceder Y","Bjartell A"],"additional_accession":[]},"is_claimable":false,"name":"Quantification of microRNA editing using two-tailed RT-qPCR for improved biomarker discovery.","description":"Even though microRNAs have been viewed as promising biomarkers for years, their clinical implementation is still lagging far behind. This is in part due to the lack of RT-qPCR technologies that can differentiate between microRNA isoforms. For example, A-to-I editing of microRNAs through adenosine deaminase acting on RNA (ADAR) enzymes can affect their expression levels and functional roles, but editing isoform-specific assays are not commercially available. Here, we describe RT-qPCR assays that are specific for editing isoforms, using microRNA-379 (miR-379) as a model. The assays are based on two-tailed RT-qPCR, and we show them to be compatible both with SYBR Green and hydrolysis-based chemistries, as well as with both qPCR and digital PCR. The assays could readily detect different miR-37","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2025-04-05T13:44:19.141Z","creation":"2025-04-05T13:44:19.141Z"},"accession":"S-EPMC8522694","cross_references":{"pubmed":["34433636"],"doi":["10.1261/rna.078867.121"]}}