biostudies-literatureUnknown3(6)Faure-Dupuy S<h4>Background & aims</h4>Immune-mediated induction of cytidine deaminase APOBEC3B (A3B) expression leads to HBV covalently closed circular DNA (cccDNA) decay. Here, we aimed to decipher the signalling pathway(s) and regulatory mechanism(s) involved in A3B induction and related HBV control.<h4>Methods</h4>Differentiated HepaRG cells (dHepaRG) knocked-down for NF-κB signalling components, transfected with siRNA or micro RNAs (miRNA), and primary human hepatocytes ± HBV or HBVΔX or HBV-RFP, were treated with lymphotoxin beta receptor (LTβR)-agonist (BS1). The biological outcomes were analysed by reverse transcriptase-qPCR, immunoblotting, luciferase activity, chromatin immune precipitation, electrophoretic mobility-shift assay, targeted-bisulfite-, miRNA-, RNA-, genome-sequencing, and mass-sJHEP reports : innovation in hepatology100354https://www.ebi.ac.uk/biostudies/studies/S-EPMC8523871biostudies-literatureControl of APOBEC3B induction and cccDNA decay by NF-κB and miR-138-5p.PMC8523871Riedl TRolland MLipka DBLucifora JFarhat RSchonung MSchuster LCHizir ZProkosch SHeikenwalder MGillet NRippe KOllinger RRad RStadler MParent RTscharahganeh DProtzer UDejardin EWettengel JDurantel DLeuchtenberger CUnger KReisinger FEngleitner TNeuhaus KSchuehle SRemouchamps CBarnault RFaure-Dupuy SfalseControl of APOBEC3B induction and cccDNA decay by NF-κB and miR-138-5p.<h4>Background & aims</h4>Immune-mediated induction of cytidine deaminase APOBEC3B (A3B) expression leads to HBV covalently closed circular DNA (cccDNA) decay. Here, we aimed to decipher the signalling pathway(s) and regulatory mechanism(s) involved in A3B induction and related HBV control.<h4>Methods</h4>Differentiated HepaRG cells (dHepaRG) knocked-down for NF-κB signalling components, transfected with siRNA or micro RNAs (miRNA), and primary human hepatocytes ± HBV or HBVΔX or HBV-RFP, were treated with lymphotoxin beta receptor (LTβR)-agonist (BS1). The biological outcomes were analysed by reverse transcriptase-qPCR, immunoblotting, luciferase activity, chromatin immune precipitation, electrophoretic mobility-shift assay, targeted-bisulfite-, miRNA-, RNA-, genome-sequencing, and mass-s2021-01-01T00:00:00Z2021 Dec2026-03-27T17:06:22.565Z2025-04-04T13:16:59.325ZS-EPMC85238713470400410.1016/j.jhepr.2021.100354