{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lin X"],"funding":["Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (NSERC)"],"pagination":["e0106821"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8525555"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6(5)"],"pubmed_abstract":["Wastewater-based genomic surveillance of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus shows promise to complement genomic epidemiology efforts. Multiplex tiling PCR is a desirable approach for targeted genome sequencing of SARS-CoV-2 in wastewater due to its low cost and rapid turnaround time. However, it is not clear how different multiplex tiling PCR primer schemes or wastewater sample matrices impact the resulting SARS-CoV-2 genome coverage. The objective of this work was to assess the performance of three different multiplex primer schemes, consisting of 150-bp, 400-bp, and 1,200-bp amplicons, as well as two wastewater sample matrices, influent wastewater and primary sludge, for targeted genome sequencing of SARS-CoV-2. Wastewater samples were collected weekly"],"journal":["mSystems"],"pubmed_title":["Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater."],"pmcid":["PMC8525555"],"funding_grant_id":["Alliance Grant"],"pubmed_authors":["Prystajecky N","Ross-Van Mierlo T","Glier M","Kuchinski K","Ziels RM","Lin X","McVea D","Tyson JR"],"additional_accession":[]},"is_claimable":false,"name":"Assessing Multiplex Tiling PCR Sequencing Approaches for Detecting Genomic Variants of SARS-CoV-2 in Municipal Wastewater.","description":"Wastewater-based genomic surveillance of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus shows promise to complement genomic epidemiology efforts. Multiplex tiling PCR is a desirable approach for targeted genome sequencing of SARS-CoV-2 in wastewater due to its low cost and rapid turnaround time. However, it is not clear how different multiplex tiling PCR primer schemes or wastewater sample matrices impact the resulting SARS-CoV-2 genome coverage. The objective of this work was to assess the performance of three different multiplex primer schemes, consisting of 150-bp, 400-bp, and 1,200-bp amplicons, as well as two wastewater sample matrices, influent wastewater and primary sludge, for targeted genome sequencing of SARS-CoV-2. Wastewater samples were collected weekly","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2026-06-17T06:23:44.085Z","creation":"2025-02-19T01:26:02.832Z"},"accession":"S-EPMC8525555","cross_references":{"pubmed":["34665013"],"doi":["10.1128/mSystems.01068-21"]}}