biostudies-literatureDey SNCATS NIH HHSNIDDK NIH HHSIndiana Clinical and Translational Sciences InstituteNCI NIH HHSIndiana University Simon Cancer Centere149539https://www.ebi.ac.uk/biostudies/studies/S-EPMC8525644biostudies-literatureUnknown6(19)MicroRNA-29 (miR-29) is a critical regulator of fibroinflammatory processes in human diseases. In this study, we found a decrease in miR-29a in experimental and human chronic pancreatitis, leading us to investigate the regulatory role of the miR-29a/b1 cluster in acute pancreatitis (AP) utilizing a conditional miR-29a/b1-KO mouse model. miR-29a/b1-sufficient (WT) and -deficient (KO) mice were administered supramaximal caerulein to induce AP and characterized at different time points, utilizing an array of IHC and biochemical analyses for AP parameters. In caerulein-induced WT mice, miR-29a remained dramatically downregulated at injury. Despite high-inflammatory milieu, fibrosis, and parenchymal disarray in the WT mice during early AP, the pancreata fully restored during recovery. miR-29a/bJCI insightLoss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis.PMC8525644U01 DK108323P30CA082709P30 CA082709UL1TR001108UL1 TR001108Easler JJPandol SDey SKota JUdari LMKwon JJFogel ELRiveraHernandez PWillis BfalseLoss of miR-29a/b1 promotes inflammation and fibrosis in acute pancreatitis.MicroRNA-29 (miR-29) is a critical regulator of fibroinflammatory processes in human diseases. In this study, we found a decrease in miR-29a in experimental and human chronic pancreatitis, leading us to investigate the regulatory role of the miR-29a/b1 cluster in acute pancreatitis (AP) utilizing a conditional miR-29a/b1-KO mouse model. miR-29a/b1-sufficient (WT) and -deficient (KO) mice were administered supramaximal caerulein to induce AP and characterized at different time points, utilizing an array of IHC and biochemical analyses for AP parameters. In caerulein-induced WT mice, miR-29a remained dramatically downregulated at injury. Despite high-inflammatory milieu, fibrosis, and parenchymal disarray in the WT mice during early AP, the pancreata fully restored during recovery. miR-29a/b2021-01-01T00:00:00Z2021 Oct2026-05-08T15:50:44.253Z2025-02-19T00:03:23.011ZS-EPMC85256443446435410.1172/jci.insight.149539