biostudies-literatureUnknown8Giddey ADNational Research FoundationDespite the availability of effective drug treatment, <i>Mycobacterium tuberculosis</i> (Mtb), the causative agent of TB disease, kills ~1. 5 million people annually, and the rising prevalence of drug resistance increasingly threatens to worsen this plight. We previously showed that sublethal exposure to the frontline anti-TB drug, rifampicin, resulted in substantial adaptive remodeling of the proteome of the model organism, <i>Mycobacterium smegmatis</i>, in the drug-sensitive mc²155 strain [wild type (WT)]. In this study, we investigate whether these responses are conserved in an engineered, isogenic mutant harboring the clinically relevant S531L rifampicin resistance-conferring mutation (SL) and distinguish the responses that are specific to RNA polymerase β subunit- (RpoB-) Frontiers in medicine723667https://www.ebi.ac.uk/biostudies/studies/S-EPMC8525676biostudies-literatureCell Wall Proteomics Reveal Phenotypic Adaption of Drug-Resistant <i>Mycobacterium smegmatis</i> to Subinhibitory Rifampicin Exposure.PMC8525676Soares NCGanief TABlackburn JMGanief NKoch AGiddey ADWarner DFfalseCell Wall Proteomics Reveal Phenotypic Adaption of Drug-Resistant <i>Mycobacterium smegmatis</i> to Subinhibitory Rifampicin Exposure.Despite the availability of effective drug treatment, <i>Mycobacterium tuberculosis</i> (Mtb), the causative agent of TB disease, kills ~1. 5 million people annually, and the rising prevalence of drug resistance increasingly threatens to worsen this plight. We previously showed that sublethal exposure to the frontline anti-TB drug, rifampicin, resulted in substantial adaptive remodeling of the proteome of the model organism, <i>Mycobacterium smegmatis</i>, in the drug-sensitive mc²155 strain [wild type (WT)]. In this study, we investigate whether these responses are conserved in an engineered, isogenic mutant harboring the clinically relevant S531L rifampicin resistance-conferring mutation (SL) and distinguish the responses that are specific to RNA polymerase β subunit- (RpoB-) 2021-01-01T00:00:00Z20212026-05-08T02:16:04.866Z2024-11-15T17:46:52.582ZS-EPMC85256763467622410.3389/fmed.2021.723667