{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fukuto A"],"funding":["NEI NIH HHS","NIEHS NIH HHS"],"pagination":["23"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8525860"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(12)"],"pubmed_abstract":["Purpose
Corneal keratocyte-fibroblast-myofibroblast (KFM) transformation plays a critical role in corneal stromal wound healing. However, the impact of engineered nanomaterials (ENMs), found in an increasing number of commercial products, on this process is poorly studied. This study investigates the effects of metal oxide ENMs on KFM transformation in vitro and in vivo.Methods
Cell viability of rabbit corneal fibroblasts (RCFs) was tested following treatment with 11 metal oxide ENMs at concentrations of 0.5 to 250 µg/ml for 24 hours. Messenger RNA (mRNA) and protein expression of αSMA, a marker of myofibroblast transformation, were measured using RCFs after exposure to 11 metal oxide ENMs at a concentration that did not affect cell viability, in media containing either 0 o"],"journal":["Translational vision science & technology"],"pubmed_title":["Metal Oxide Engineered Nanomaterials Modulate Rabbit Corneal Fibroblast to Myofibroblast Transformation."],"pmcid":["PMC8525860"],"funding_grant_id":["U24 ES026946","U01 ES027288","P30 ES023513","R01 EY019970","P30 EY012576"],"pubmed_authors":["Kim S","Leonard BC","Gates BL","Murphy CJ","Kiuchi Y","Thomasy SM","Van Winkle LS","Fukuto A","Kang J","Pinkerton KE","Chang MW"],"additional_accession":[]},"is_claimable":false,"name":"Metal Oxide Engineered Nanomaterials Modulate Rabbit Corneal Fibroblast to Myofibroblast Transformation.","description":"Purpose
Corneal keratocyte-fibroblast-myofibroblast (KFM) transformation plays a critical role in corneal stromal wound healing. However, the impact of engineered nanomaterials (ENMs), found in an increasing number of commercial products, on this process is poorly studied. This study investigates the effects of metal oxide ENMs on KFM transformation in vitro and in vivo.Methods
Cell viability of rabbit corneal fibroblasts (RCFs) was tested following treatment with 11 metal oxide ENMs at concentrations of 0.5 to 250 µg/ml for 24 hours. Messenger RNA (mRNA) and protein expression of αSMA, a marker of myofibroblast transformation, were measured using RCFs after exposure to 11 metal oxide ENMs at a concentration that did not affect cell viability, in media containing either 0 o","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2025-04-19T06:07:12.655Z","creation":"2025-04-19T06:07:12.655Z"},"accession":"S-EPMC8525860","cross_references":{"pubmed":["34661622"],"doi":["10.1167/tvst.10.12.23"]}}