{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["17(10)"],"submitter":["Teper-Bamnolker P"],"pubmed_abstract":["The caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Under conditions of carbon starvation, VPE activity and PCD symptoms strongly increased in BY-2 cells, but to a much lesser extent in <i>VPE</i>-RNAi BY-2 cells. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole through tonoplast engulfment. Exposure of BY-2 culture to the macroautophagy"],"journal":["Autophagy"],"pagination":["3109-3123"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8526000"],"repository":["biostudies-literature"],"pubmed_title":["Vacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death."],"pmcid":["PMC8526000"],"pubmed_authors":["Avin-Wittenberg T","Sadot E","Eshel D","Teper-Bamnolker P","Danieli R","Belausov E","Abu-Abied M","Peled-Zehavi H"],"additional_accession":[]},"is_claimable":false,"name":"Vacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death.","description":"The caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Under conditions of carbon starvation, VPE activity and PCD symptoms strongly increased in BY-2 cells, but to a much lesser extent in <i>VPE</i>-RNAi BY-2 cells. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole through tonoplast engulfment. Exposure of BY-2 culture to the macroautophagy","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2026-05-31T19:54:09.835Z","creation":"2025-04-05T22:20:01.751Z"},"accession":"S-EPMC8526000","cross_references":{"pubmed":["33249982"],"doi":["10.1080/15548627.2020.1856492"]}}