biostudies-literatureUnknown17(10)Teper-Bamnolker PThe caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Under conditions of carbon starvation, VPE activity and PCD symptoms strongly increased in BY-2 cells, but to a much lesser extent in <i>VPE</i>-RNAi BY-2 cells. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole through tonoplast engulfment. Exposure of BY-2 culture to the macroautophagyAutophagy3109-3123https://www.ebi.ac.uk/biostudies/studies/S-EPMC8526000biostudies-literatureVacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death.PMC8526000Avin-Wittenberg TSadot EEshel DTeper-Bamnolker PDanieli RBelausov EAbu-Abied MPeled-Zehavi HfalseVacuolar processing enzyme translocates to the vacuole through the autophagy pathway to induce programmed cell death.The caspase-like vacuolar processing enzyme (VPE) is a key factor in programmed cell death (PCD) associated with plant stress responses. Growth medium lacking a carbon source and dark conditions caused punctate labeling of 35S::VPE1-GFP (StVPE1-GFP) in potato leaves. Under conditions of carbon starvation, VPE activity and PCD symptoms strongly increased in BY-2 cells, but to a much lesser extent in <i>VPE</i>-RNAi BY-2 cells. During extended exposure to carbon starvation, VPE expression and activity levels peaked, with a gradual increase in BY-2 cell death. Histological analysis of StVPE1-GFP in BY-2 cells showed that carbon starvation induces its translocation from the endoplasmic reticulum to the central vacuole through tonoplast engulfment. Exposure of BY-2 culture to the macroautophagy2021-01-01T00:00:00Z2021 Oct2026-05-31T19:54:09.835Z2025-04-05T22:20:01.751ZS-EPMC85260003324998210.1080/15548627.2020.1856492