biostudies-literatureD'Souza ANCI NIH HHSNINDS NIH HHSNIGMS NIH HHS505-526https://www.ebi.ac.uk/biostudies/studies/S-EPMC8526414biostudies-literatureUnknown338We have demonstrated, for the first time that microvesicles, a sub-type of extracellular vesicles (EVs) derived from hCMEC/D3: a human brain endothelial cell (BEC) line transfer polarized mitochondria to recipient BECs in culture and to neurons in mice acute brain cortical and hippocampal slices. This mitochondrial transfer increased ATP levels by 100 to 200-fold (relative to untreated cells) in the recipient BECs exposed to oxygen-glucose deprivation, an in vitro model of cerebral ischemia. We have also demonstrated that transfer of microvesicles, the larger EV fraction, but not exosomes resulted in increased mitochondrial function in hypoxic endothelial cultures. Gene ontology and pathway enrichment analysis of EVs revealed a very high association to glycolysis-related processes. In compJournal of controlled release : official journal of the Controlled Release SocietyMicrovesicles transfer mitochondria and increase mitochondrial function in brain endothelial cells.PMC8526414P30 CA047904R25 NS100118T32 GM007635Reynolds MJShiva SSDobbins DXQuillinan NSabatelle CManickam DSZhao WJoy GMSoman VD'Souza ADave KMHerson PSChandran URBurch AKamte YSSreeram AfalseMicrovesicles transfer mitochondria and increase mitochondrial function in brain endothelial cells.We have demonstrated, for the first time that microvesicles, a sub-type of extracellular vesicles (EVs) derived from hCMEC/D3: a human brain endothelial cell (BEC) line transfer polarized mitochondria to recipient BECs in culture and to neurons in mice acute brain cortical and hippocampal slices. This mitochondrial transfer increased ATP levels by 100 to 200-fold (relative to untreated cells) in the recipient BECs exposed to oxygen-glucose deprivation, an in vitro model of cerebral ischemia. We have also demonstrated that transfer of microvesicles, the larger EV fraction, but not exosomes resulted in increased mitochondrial function in hypoxic endothelial cultures. Gene ontology and pathway enrichment analysis of EVs revealed a very high association to glycolysis-related processes. In comp2021-01-01T00:00:00Z2021 Oct2026-06-01T02:11:10.914Z2025-02-19T01:27:48.084ZS-EPMC85264143445019610.1016/j.jconrel.2021.08.038