{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Togano S"],"funding":["KAKENHI Grant-in-Aid for Scientific Research"],"pagination":["20664"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8526688"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(1)"],"pubmed_abstract":["Cancer stem cells (CSCs) play an important role in the progression of carcinoma and have a high potential for survival in stress environments. However, the mechanisms of survival potential of CSCs have been unclear. The aim of this study was to clarify the significance of autophagy systems of CSCs under stress environments. Four gastric cancer cell line were used. Side population (SP) cells were sorted from the parent cells, as CSC rich cells. The expression of stem cell markers was examined by RT-PCR. The viability of cancer cells under starvation and hypoxia was evaluated. The expression level of the autophagy molecule LC3B-II was examined by western blot. The numbers of autophagosomes and autolysosomes were counted by electron microscope. SP cells of OCUM-12 showed a higher expression o"],"journal":["Scientific reports"],"pubmed_title":["Gastric cancer stem cells survive in stress environments via their autophagy system."],"pmcid":["PMC8526688"],"funding_grant_id":["18H02883(M.Y.)"],"pubmed_authors":["Sugimoto A","Kushiyama S","Sera T","Yamamoto Y","Yashiro M","Masuda G","Nishimura S","Okuno T","Kuroda K","Togano S","Miki Y","Ohira M"],"additional_accession":[]},"is_claimable":false,"name":"Gastric cancer stem cells survive in stress environments via their autophagy system.","description":"Cancer stem cells (CSCs) play an important role in the progression of carcinoma and have a high potential for survival in stress environments. However, the mechanisms of survival potential of CSCs have been unclear. The aim of this study was to clarify the significance of autophagy systems of CSCs under stress environments. Four gastric cancer cell line were used. Side population (SP) cells were sorted from the parent cells, as CSC rich cells. The expression of stem cell markers was examined by RT-PCR. The viability of cancer cells under starvation and hypoxia was evaluated. The expression level of the autophagy molecule LC3B-II was examined by western blot. The numbers of autophagosomes and autolysosomes were counted by electron microscope. SP cells of OCUM-12 showed a higher expression o","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2026-05-08T02:17:55.703Z","creation":"2025-04-19T08:44:55.679Z"},"accession":"S-EPMC8526688","cross_references":{"pubmed":["34667215"],"doi":["10.1038/s41598-021-00155-3"]}}