biostudies-literatureMurphy NAgencia Nacional de Promoción Científica y TecnológicaUniversidad de Buenos AiresSir Halley Stewart TrustFundación Bunge y Born543https://www.ebi.ac.uk/biostudies/studies/S-EPMC8527601biostudies-literatureUnknown14(1)<h4>Background</h4>Chagas disease remains a significant public health problem in Latin America. There are only two chemotherapy drugs, nifurtimox and benznidazole, and both may have severe side effects. After complete chemotherapy of acute cases, seropositive diagnosis may revert to negative. However, there are no definitive parasitological or serological biomarkers of cure.<h4>Methods</h4>Following a pilot study with seven Bolivian migrants to Spain, we tested 71 serum samples from chronic patients (mean age 12.6 years) inhabiting the Argentine Chaco region. Benznidazole chemotherapy (5-8 mg/kg day, twice daily for 60 days) was administered during 2011-2016. Subsequently, pre-and post-chemotherapy serum samples were analysed in pairs by IgG1 and IgG ELISA using two different antigens and Parasites & vectorsAssessing antibody decline after chemotherapy of early chronic Chagas disease patients.PMC8527601UBACYT20020170100779BA)UBACYT 20020130100843BAPICT 2015-2921)Cardinal MVFreilij HBhattacharyya TAlvedro AGurtler REMertens PMiles MAMartinez de Salazar PMolina IEnriquez GFMurphy NMacchiaverna NPGilleman QfalseAssessing antibody decline after chemotherapy of early chronic Chagas disease patients.<h4>Background</h4>Chagas disease remains a significant public health problem in Latin America. There are only two chemotherapy drugs, nifurtimox and benznidazole, and both may have severe side effects. After complete chemotherapy of acute cases, seropositive diagnosis may revert to negative. However, there are no definitive parasitological or serological biomarkers of cure.<h4>Methods</h4>Following a pilot study with seven Bolivian migrants to Spain, we tested 71 serum samples from chronic patients (mean age 12.6 years) inhabiting the Argentine Chaco region. Benznidazole chemotherapy (5-8 mg/kg day, twice daily for 60 days) was administered during 2011-2016. Subsequently, pre-and post-chemotherapy serum samples were analysed in pairs by IgG1 and IgG ELISA using two different antigens and 2021-01-01T00:00:00Z2021 Oct2025-04-04T09:18:46.998Z2025-04-04T09:18:46.998ZS-EPMC85276013467060210.1186/s13071-021-05040-6