biostudies-literatureSchardt JSU.S. Department of Health & Human Services | National Institutes of HealthU.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)NIA NIH HHSU.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious DiseasesNIAID NIH HHSAlbert M. Mattocks Chair, Biointerfaces InstituteUniversity of Michigan Institutional FundsNational Science Foundation (NSF)COVID-19: CVC Impact Research Ignitor Grant AwardUniversity of Michigan MICHR Accelerating Synergy AwardMICHR Education PTSP 2020NSF Graduate Research FellowshipU.S. Department of Health & Human Services | National Institutes of Health (NIH)U.S. Department of Health & Human Services | NIH | National Institute of General Medical SciencesNIGMS NIH HHSNational Science Foundation20738https://www.ebi.ac.uk/biostudies/studies/S-EPMC8528929biostudies-literatureUnknown11(1)Monoclonal antibodies that target SARS-CoV-2 with high affinity are valuable for a wide range of biomedical applications involving novel coronavirus disease (COVID-19) diagnosis, treatment, and prophylactic intervention. Strategies for the rapid and reliable isolation of these antibodies, especially potent neutralizing antibodies, are critical toward improved COVID-19 response and informed future response to emergent infectious diseases. In this study, single B cell screening was used to interrogate antibody repertoires of immunized mice and isolate antigen-specific IgG1⁺ memory B cells. Using these methods, high-affinity, potent neutralizing antibodies were identified that target the receptor-binding domain of SARS-CoV-2. Further engineering of the identified molecules to increScientific reportsDiscovery and characterization of high-affinity, potent SARS-CoV-2 neutralizing antibodies via single B cell screening.PMC8528929R35 GM1363001159943F32 GM137513RF1AG059723R01 AI 51588 0118139631605266RF1 AG059723R01 AI051588R35GM136300Lanigan TMZupancic JMMakowski EKGarcia de Mattos Barbosa MTessier PMMoon JJChen HSchardt JSPark KSDesai AAPornnoppadol GCascalho MSmith MDfalseDiscovery and characterization of high-affinity, potent SARS-CoV-2 neutralizing antibodies via single B cell screening.Monoclonal antibodies that target SARS-CoV-2 with high affinity are valuable for a wide range of biomedical applications involving novel coronavirus disease (COVID-19) diagnosis, treatment, and prophylactic intervention. Strategies for the rapid and reliable isolation of these antibodies, especially potent neutralizing antibodies, are critical toward improved COVID-19 response and informed future response to emergent infectious diseases. In this study, single B cell screening was used to interrogate antibody repertoires of immunized mice and isolate antigen-specific IgG1⁺ memory B cells. Using these methods, high-affinity, potent neutralizing antibodies were identified that target the receptor-binding domain of SARS-CoV-2. Further engineering of the identified molecules to incre2021-01-01T00:00:00Z2021 Oct2025-04-04T09:18:39.07Z2025-04-04T09:18:39.07ZS-EPMC85289293467108010.1038/s41598-021-99401-x