{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pradhan AJ"],"funding":["NCATS NIH HHS","NIDCR NIH HHS","National Institutes of Health","NIH HHS","State University of New York","NIGMS NIH HHS","National Science Foundation"],"pagination":["1298-1309.e7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8529612"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(9)"],"pubmed_abstract":["Necroptosis is a form of cell death characterized by receptor-interacting protein kinase activity and plasma membrane permeabilization via mixed-lineage kinase-like protein (MLKL). This permeabilization is responsible for the inflammatory properties of necroptosis. We previously showed that very long chain fatty acids (VLCFAs) are functionally involved in necroptosis, potentially through protein fatty acylation. Here, we define the scope of protein acylation by saturated VLCFAs during necroptosis. We show that MLKL and phosphoMLKL, key for membrane permeabilization, are exclusively acylated during necroptosis. Reducing the levels of VLCFAs decreases their membrane recruitment, suggesting that acylation by VLCFAs contributes to their membrane localization. Acylation of phosphoMLKL occurs do"],"journal":["Cell chemical biology"],"pubmed_title":["Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis."],"pmcid":["PMC8529612"],"funding_grant_id":["R25 GM095459","R01 GM078383","DE028307","R01 DE028307","GM095459","MCB1817468","UL1 TR001412","S10 OD025204","GM078383"],"pubmed_authors":["Bynum K","Chemler SR","Parisi LR","Berhane IA","Qu J","Lu D","Kay JG","Galster SL","Pradhan AJ","Gokcumen O","Monje-Galvan V","Shen S","Atilla-Gokcumen GE"],"additional_accession":[]},"is_claimable":false,"name":"Protein acylation by saturated very long chain fatty acids and endocytosis are involved in necroptosis.","description":"Necroptosis is a form of cell death characterized by receptor-interacting protein kinase activity and plasma membrane permeabilization via mixed-lineage kinase-like protein (MLKL). This permeabilization is responsible for the inflammatory properties of necroptosis. We previously showed that very long chain fatty acids (VLCFAs) are functionally involved in necroptosis, potentially through protein fatty acylation. Here, we define the scope of protein acylation by saturated VLCFAs during necroptosis. We show that MLKL and phosphoMLKL, key for membrane permeabilization, are exclusively acylated during necroptosis. Reducing the levels of VLCFAs decreases their membrane recruitment, suggesting that acylation by VLCFAs contributes to their membrane localization. Acylation of phosphoMLKL occurs do","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2026-05-08T02:01:45.741Z","creation":"2025-02-18T23:55:46.725Z"},"accession":"S-EPMC8529612","cross_references":{"pubmed":["33848465"],"doi":["10.1016/j.chembiol.2021.03.012"]}}