biostudies-literatureUnknown12Fukushi IAcute hypoxia increases ventilation. After cessation of hypoxia loading, ventilation decreases but remains above the pre-exposure baseline level for a time. However, the mechanism of this post-hypoxic persistent respiratory augmentation (PHRA), which is a short-term potentiation of breathing, has not been elucidated. We aimed to test the hypothesis that astrocytes are involved in PHRA. To this end, we investigated hypoxic ventilatory responses by whole-body plethysmography in unanesthetized adult mice. The animals breathed room air, hypoxic gas mixture (7% O₂, 93% N₂) for 2min, and again room air for 10min before and after i.p. administration of low (100mg/kg) and high (300mg/kg) doses of arundic acid (AA), an astrocyte inhibitor. AA suppressed PHRA, with the high dosFrontiers in physiology757731https://www.ebi.ac.uk/biostudies/studies/S-EPMC8531090biostudies-literatureActivation of Astrocytes in the Persistence of Post-hypoxic Respiratory Augmentation.PMC8531090Yoshizawa MFukushi IOnimaru HOkada YMori YKono YNakao ATakeda KHasebe YPokorski MfalseActivation of Astrocytes in the Persistence of Post-hypoxic Respiratory Augmentation.Acute hypoxia increases ventilation. After cessation of hypoxia loading, ventilation decreases but remains above the pre-exposure baseline level for a time. However, the mechanism of this post-hypoxic persistent respiratory augmentation (PHRA), which is a short-term potentiation of breathing, has not been elucidated. We aimed to test the hypothesis that astrocytes are involved in PHRA. To this end, we investigated hypoxic ventilatory responses by whole-body plethysmography in unanesthetized adult mice. The animals breathed room air, hypoxic gas mixture (7% O₂, 93% N₂) for 2min, and again room air for 10min before and after i.p. administration of low (100mg/kg) and high (300mg/kg) doses of arundic acid (AA), an astrocyte inhibitor. AA suppressed PHRA, with the high dos2021-01-01T00:00:00Z20212025-04-22T20:50:15.572Z2025-04-06T03:18:15.557ZS-EPMC85310903469082010.3389/fphys.2021.757731