{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Meng X"],"funding":["European Research Council","UK Research and Innovation","Engineering and Physical Sciences Research Council"],"pagination":["3111-3118"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8532162"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(10)"],"pubmed_abstract":["Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterization of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background environments, such as tracking lipids or proteins in or on cells, due to superior background rejection. Scattering-based detection is preferable when localization precision and imaging speed are paramount due to the in principle infinite photon budget. Here, we show that micromirror-based total internal reflection dark field microscopy enables background suppression previously only reported for interferometric scattering microscopy, resulting in nanometer localization precision at 6 μs exposure time for 20 nm gold nanoparticles with a 25 × 25 μm<sup>2</sup> field of view. We demonstrate the capabilities of our implementation by characterizing sub-nanometer deterministic flows of 20 nm gold nanoparticles at liquid-liquid interfaces. Our results approach the optimal combination of background suppression, localization precision, and temporal resolution achievable with pure scattering-based imaging and tracking of nanoparticles at interfaces."],"journal":["ACS photonics"],"pubmed_title":["Micromirror Total Internal Reflection Microscopy for High-Performance Single Particle Tracking at Interfaces."],"pmcid":["PMC8532162"],"funding_grant_id":["819593","EP/T03419X/1"],"pubmed_authors":["Thorpe S","Sonn-Segev A","Dufresne ER","Young G","Cole D","Style RW","Meng X","Kukura P","Schumacher A"],"additional_accession":[]},"is_claimable":false,"name":"Micromirror Total Internal Reflection Microscopy for High-Performance Single Particle Tracking at Interfaces.","description":"Single particle tracking has found broad applications in the life and physical sciences, enabling the observation and characterization of nano- and microscopic motion. Fluorescence-based approaches are ideally suited for high-background environments, such as tracking lipids or proteins in or on cells, due to superior background rejection. Scattering-based detection is preferable when localization precision and imaging speed are paramount due to the in principle infinite photon budget. Here, we show that micromirror-based total internal reflection dark field microscopy enables background suppression previously only reported for interferometric scattering microscopy, resulting in nanometer localization precision at 6 μs exposure time for 20 nm gold nanoparticles with a 25 × 25 μm<sup>2</sup> field of view. We demonstrate the capabilities of our implementation by characterizing sub-nanometer deterministic flows of 20 nm gold nanoparticles at liquid-liquid interfaces. Our results approach the optimal combination of background suppression, localization precision, and temporal resolution achievable with pure scattering-based imaging and tracking of nanoparticles at interfaces.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2025-04-22T07:53:06.893Z","creation":"2025-04-05T22:20:37.757Z"},"accession":"S-EPMC8532162","cross_references":{"pubmed":["34692901"],"doi":["10.1021/acsphotonics.1c01268"]}}