biostudies-literatureIslam MSKobayashi FoundationThe Smoking Research Foundation of JapanThe Grant of National Center for Child Health and DevelopmentJapan Society for the Promotion of Science2876https://www.ebi.ac.uk/biostudies/studies/S-EPMC8532638biostudies-literatureUnknown11(10)17β-hydroxysteroid dehydrogenase type 3 (HSD17B3) converts androstenedione (A4) into testosterone (T), which regulates sex steroid production. Because various mutations of the HSD17B3 gene cause disorder of sex differentiation (DSD) in multiple mammalian species, it is very important to reveal the molecular characteristics of this gene in various species. Here, we revealed the open reading frame of the ovine HSD17B3 gene. Enzymatic activities of ovine HSD17B3 and HSD17B1 for converting A4 to T were detected using ovine androgen receptor-mediated transactivation in reporter assays. Although HSD17B3 also converted estrone to estradiol, this activity was much weaker than those of HSD17B1. Although ovine HSD17B3 has an amino acid sequence that is conserved compared with other mammalian speciesAnimals : an open access journal from MDPIAnalyses of Molecular Characteristics and Enzymatic Activities of Ovine HSD17B3.PMC853263819K097942152020G0062021-A118119K07117Kitano TSuzuki NIda TIslam MSUwada JYazawa TKikuya KIYaegashi KSato THayashi JMiyashiro YUmezawa ATakahashi SWatanabe HSekiguchi TKhan RIImamichi YMuranishi YHasegawa KfalseAnalyses of Molecular Characteristics and Enzymatic Activities of Ovine HSD17B3.17β-hydroxysteroid dehydrogenase type 3 (HSD17B3) converts androstenedione (A4) into testosterone (T), which regulates sex steroid production. Because various mutations of the HSD17B3 gene cause disorder of sex differentiation (DSD) in multiple mammalian species, it is very important to reveal the molecular characteristics of this gene in various species. Here, we revealed the open reading frame of the ovine HSD17B3 gene. Enzymatic activities of ovine HSD17B3 and HSD17B1 for converting A4 to T were detected using ovine androgen receptor-mediated transactivation in reporter assays. Although HSD17B3 also converted estrone to estradiol, this activity was much weaker than those of HSD17B1. Although ovine HSD17B3 has an amino acid sequence that is conserved compared with other mammalian species2021-01-01T00:00:00Z2021 Sep2026-05-04T03:08:47.492Z2024-11-08T11:39:45.859ZS-EPMC85326383467989710.3390/ani11102876