biostudies-literatureViau MInstitut National Du CancerAgence Nationale de la RechercheCommissariat Général à l'InvestissementCancéropôle CLARA1462https://www.ebi.ac.uk/biostudies/studies/S-EPMC8533583biostudies-literatureUnknown11(10)Despite a considerable amount of data, the molecular and cellular bases of the toxicity due to metal exposure remain unknown. Recent mechanistic models from radiobiology have emerged, pointing out that the radiation-induced nucleo-shuttling of the ATM protein (RIANS) initiates the recognition and the repair of DNA double-strand breaks (DSB) and the final response to genotoxic stress. In order to document the role of ATM-dependent DSB repair and signalling after metal exposure, we applied twelve different metal species representing nine elements (Al, Cu, Zn Ni, Pd, Cd, Pb, Cr, and Fe) to human skin, mammary, and brain cells. Our findings suggest that metals may directly or indirectly induce DSB at a rate that depends on the metal properties and concentration, and tissue type. At specific meBiomoleculesDNA Double-Strand Breaks Induced in Human Cells by Twelve Metallic Species: Quantitative Inter-Comparisons and Influence of the ATM Protein.PMC8533583INDIRA ProjectEURIPIDE ProjectPROUST ProjectHEMI-BREAKS ProjectFervers BViau MBerthel EBodgi LDevic CPereira SFerlazzo MLCharlet LSonzogni LForay NGranzotto AfalseDNA Double-Strand Breaks Induced in Human Cells by Twelve Metallic Species: Quantitative Inter-Comparisons and Influence of the ATM Protein.Despite a considerable amount of data, the molecular and cellular bases of the toxicity due to metal exposure remain unknown. Recent mechanistic models from radiobiology have emerged, pointing out that the radiation-induced nucleo-shuttling of the ATM protein (RIANS) initiates the recognition and the repair of DNA double-strand breaks (DSB) and the final response to genotoxic stress. In order to document the role of ATM-dependent DSB repair and signalling after metal exposure, we applied twelve different metal species representing nine elements (Al, Cu, Zn Ni, Pd, Cd, Pb, Cr, and Fe) to human skin, mammary, and brain cells. Our findings suggest that metals may directly or indirectly induce DSB at a rate that depends on the metal properties and concentration, and tissue type. At specific me2021-01-01T00:00:00Z2021 Oct2025-04-21T21:59:13.763Z2025-04-21T21:59:13.763ZS-EPMC85335833468009510.3390/biom11101462