{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mahmoudi T"],"funding":["Tabriz University of Medical Sciences","The Iran National Science Foundation"],"pagination":["392"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8534016"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(10)"],"pubmed_abstract":["Real-time connectivity and employment of sustainable materials empowers point-of-care diagnostics with the capability to send clinically relevant data to health care providers even in low-resource settings. In this study, we developed an advantageous kit for the on-site detection of carcinoembryonic antigen (CEA) in human serum. CEA sensing was performed using cellulose-based lateral flow strips, and colorimetric signals were read, processed, and measured using a smartphone-based system. The corresponding immunoreaction was reported by polydopamine-modified gold nanoparticles in order to boost the signal intensity and improve the surface blocking and signal-to-noise relationship, thereby enhancing detection sensitivity when compared with bare gold nanoparticles (up to 20-fold in terms of visual limit of detection). Such lateral flow strips showed a linear range from 0.05 to 50 ng/mL, with a visual limit of detection of 0.05 ng/mL and an assay time of 15 min. Twenty-six clinical samples were also tested using the proposed kit and compared with the gold standard of immunoassays (enzyme linked immunosorbent assay), demonstrating an excellent correlation (R = 0.99). This approach can potentially be utilized for the monitoring of cancer treatment, particularly at locations far from centralized laboratory facilities."],"journal":["Biosensors"],"pubmed_title":["On-Site Detection of Carcinoembryonic Antigen in Human Serum."],"pmcid":["PMC8534016"],"funding_grant_id":["97001910","60836 and 63253"],"pubmed_authors":["Golmohammadi H","Mahmoudi T","Baradaran B","Pourhassan-Moghaddam M","Morales-Narvaez E","Shirdel B"],"additional_accession":[]},"is_claimable":false,"name":"On-Site Detection of Carcinoembryonic Antigen in Human Serum.","description":"Real-time connectivity and employment of sustainable materials empowers point-of-care diagnostics with the capability to send clinically relevant data to health care providers even in low-resource settings. In this study, we developed an advantageous kit for the on-site detection of carcinoembryonic antigen (CEA) in human serum. CEA sensing was performed using cellulose-based lateral flow strips, and colorimetric signals were read, processed, and measured using a smartphone-based system. The corresponding immunoreaction was reported by polydopamine-modified gold nanoparticles in order to boost the signal intensity and improve the surface blocking and signal-to-noise relationship, thereby enhancing detection sensitivity when compared with bare gold nanoparticles (up to 20-fold in terms of visual limit of detection). Such lateral flow strips showed a linear range from 0.05 to 50 ng/mL, with a visual limit of detection of 0.05 ng/mL and an assay time of 15 min. Twenty-six clinical samples were also tested using the proposed kit and compared with the gold standard of immunoassays (enzyme linked immunosorbent assay), demonstrating an excellent correlation (R = 0.99). This approach can potentially be utilized for the monitoring of cancer treatment, particularly at locations far from centralized laboratory facilities.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2025-04-04T13:16:57.603Z","creation":"2025-04-04T13:16:57.603Z"},"accession":"S-EPMC8534016","cross_references":{"pubmed":["34677348"],"doi":["10.3390/bios11100392"]}}