{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["11(10)"],"submitter":["Lownik JC"],"pubmed_abstract":["SARS-CoV-2, the virus responsible for COVID-19, emerged in late 2019 and has since spread throughout the world, infecting over 200 million people. The fast spread of SARS-CoV-2 showcased the need for rapid and sensitive testing methodologies to help track the disease. Over the past 18 months, numerous SARS-CoV-2 variants have emerged. Many of these variants are suggested to be more transmissible as well as less responsive to neutralization by vaccine-induced antibodies. Viral whole-genome sequencing is the current standard for tracking these variants. However, whole-genome sequencing is costly and the technology and expertise are limited to larger reference laboratories. Here, we present the feasibility of a fast, inexpensive methodology using snapback primer-based high-resolution melting to test for >20 high-consequence SARS-CoV-2 spike mutations. This assay can distinguish between multiple variant lineages and be completed in roughly 2 h for less than $10 per sample."],"journal":["Diagnostics (Basel, Switzerland)"],"pagination":["1788"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8534650"],"repository":["biostudies-literature"],"pubmed_title":["Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting."],"pmcid":["PMC8534650"],"pubmed_authors":["McKay A","Martin RK","Way GW","Roychoudhury P","Lownik JC","Greninger AL","Farrar JS"],"additional_accession":[]},"is_claimable":false,"name":"Fast SARS-CoV-2 Variant Detection Using Snapback Primer High-Resolution Melting.","description":"SARS-CoV-2, the virus responsible for COVID-19, emerged in late 2019 and has since spread throughout the world, infecting over 200 million people. The fast spread of SARS-CoV-2 showcased the need for rapid and sensitive testing methodologies to help track the disease. Over the past 18 months, numerous SARS-CoV-2 variants have emerged. Many of these variants are suggested to be more transmissible as well as less responsive to neutralization by vaccine-induced antibodies. Viral whole-genome sequencing is the current standard for tracking these variants. However, whole-genome sequencing is costly and the technology and expertise are limited to larger reference laboratories. Here, we present the feasibility of a fast, inexpensive methodology using snapback primer-based high-resolution melting to test for >20 high-consequence SARS-CoV-2 spike mutations. This assay can distinguish between multiple variant lineages and be completed in roughly 2 h for less than $10 per sample.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2026-05-03T03:15:50.292Z","creation":"2025-02-19T00:28:05.731Z"},"accession":"S-EPMC8534650","cross_references":{"pubmed":["34679489"],"doi":["10.3390/diagnostics11101788"]}}