{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pi Y"],"funding":["National Key Basic Research Program of China","National Natural Science Foundation of China"],"pagination":["e00176-20"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8534727"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(3)"],"pubmed_abstract":["Dietary high protein and low carbohydrate levels compromise colonic microbiota and bile acid metabolism, which underlies a detrimental gut environment. However, it remains unclear if the diet-induced changes in colonic health are due to a change in hindgut nutrient availability and what key intermediates link the microbe-epithelium dialogue. To specifically alter the hindgut nutrient substrate availability, here we used a cecally cannulated pig model to infuse corn starch and casein hydrolysate directly into the cecum to generate a stepwise change of carbohydrate/nitrogenous compound (C/N) ratio. Pigs were cecally infused daily with either saline (Control), corn starch (Starch), or casein hydrolysate (Casein) (<i>n</i> = 8 per group), respectively, for 19 days. After infusion, C/N ratios i"],"journal":["mSystems"],"pubmed_title":["Increasing the Hindgut Carbohydrate/Protein Ratio by Cecal Infusion of Corn Starch or Casein Hydrolysate Drives Gut Microbiota-Related Bile Acid Metabolism To Stimulate Colonic Barrier Function."],"pmcid":["PMC8534727"],"funding_grant_id":["31430082","2013CB127300"],"pubmed_authors":["Peng Y","Liu Z","Gao K","Pi Y","Zhu W","Mu C"],"additional_accession":[]},"is_claimable":false,"name":"Increasing the Hindgut Carbohydrate/Protein Ratio by Cecal Infusion of Corn Starch or Casein Hydrolysate Drives Gut Microbiota-Related Bile Acid Metabolism To Stimulate Colonic Barrier Function.","description":"Dietary high protein and low carbohydrate levels compromise colonic microbiota and bile acid metabolism, which underlies a detrimental gut environment. However, it remains unclear if the diet-induced changes in colonic health are due to a change in hindgut nutrient availability and what key intermediates link the microbe-epithelium dialogue. To specifically alter the hindgut nutrient substrate availability, here we used a cecally cannulated pig model to infuse corn starch and casein hydrolysate directly into the cecum to generate a stepwise change of carbohydrate/nitrogenous compound (C/N) ratio. Pigs were cecally infused daily with either saline (Control), corn starch (Starch), or casein hydrolysate (Casein) (<i>n</i> = 8 per group), respectively, for 19 days. After infusion, C/N ratios i","dates":{"release":"2020-01-01T00:00:00Z","publication":"2020 Jun","modification":"2026-06-17T06:23:59.345Z","creation":"2025-02-19T01:26:04.611Z"},"accession":"S-EPMC8534727","cross_references":{"pubmed":["32487741"],"doi":["10.1128/mSystems.00176-20"]}}