{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(10)"],"submitter":["Cazzato G"],"pubmed_abstract":["Over the years, increasing information has been asked of the pathologist: we have moved from a purely morphological diagnosis to biomolecular and genetic studies, which have made it possible to implement the use of molecular targeted therapies, such as anti-epidermal growth factor receptor (EGFR) molecules in EGFR-mutated lung cancer, for example. Today, next generation sequencing (NGS) has changed the approach to neoplasms, to the extent that, in a short time, it has gained a place of absolute importance and diagnostic, prognostic and therapeutic utility. In this scenario, formaldehyde-fixed and paraffin-embedded (FFPE) biological tissue samples are a source of clinical and molecular information. However, problems can arise in the genetic material (DNA and RNA) for use in NGS due to fixat"],"journal":["Genes"],"pagination":["1472"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8535326"],"repository":["biostudies-literature"],"pubmed_title":["Formalin-Fixed and Paraffin-Embedded Samples for Next Generation Sequencing: Problems and Solutions."],"pmcid":["PMC8535326"],"pubmed_authors":["Cimmino A","Romita P","Foti C","Venerito V","Cormio G","Maiorano E","Cazzato G","Caporusso C","Rossi R","De Marco A","Colagrande A","Loizzi V","Parente P","Ingravallo G","Scarcella VS","Lettini T","Stellacci A","Marrone M","Resta L","Tarantino P","Arezzo F"],"additional_accession":[]},"is_claimable":false,"name":"Formalin-Fixed and Paraffin-Embedded Samples for Next Generation Sequencing: Problems and Solutions.","description":"Over the years, increasing information has been asked of the pathologist: we have moved from a purely morphological diagnosis to biomolecular and genetic studies, which have made it possible to implement the use of molecular targeted therapies, such as anti-epidermal growth factor receptor (EGFR) molecules in EGFR-mutated lung cancer, for example. Today, next generation sequencing (NGS) has changed the approach to neoplasms, to the extent that, in a short time, it has gained a place of absolute importance and diagnostic, prognostic and therapeutic utility. In this scenario, formaldehyde-fixed and paraffin-embedded (FFPE) biological tissue samples are a source of clinical and molecular information. However, problems can arise in the genetic material (DNA and RNA) for use in NGS due to fixat","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-21T14:51:17.062Z","creation":"2025-04-21T14:51:17.062Z"},"accession":"S-EPMC8535326","cross_references":{"pubmed":["34680867"],"doi":["10.3390/genes12101472"]}}