biostudies-literatureUnknown12(10)Cazzato GOver the years, increasing information has been asked of the pathologist: we have moved from a purely morphological diagnosis to biomolecular and genetic studies, which have made it possible to implement the use of molecular targeted therapies, such as anti-epidermal growth factor receptor (EGFR) molecules in EGFR-mutated lung cancer, for example. Today, next generation sequencing (NGS) has changed the approach to neoplasms, to the extent that, in a short time, it has gained a place of absolute importance and diagnostic, prognostic and therapeutic utility. In this scenario, formaldehyde-fixed and paraffin-embedded (FFPE) biological tissue samples are a source of clinical and molecular information. However, problems can arise in the genetic material (DNA and RNA) for use in NGS due to fixatGenes1472https://www.ebi.ac.uk/biostudies/studies/S-EPMC8535326biostudies-literatureFormalin-Fixed and Paraffin-Embedded Samples for Next Generation Sequencing: Problems and Solutions.PMC8535326Cimmino ARomita PFoti CVenerito VCormio GMaiorano ECazzato GCaporusso CRossi RDe Marco AColagrande ALoizzi VParente PIngravallo GScarcella VSLettini TStellacci AMarrone MResta LTarantino PArezzo FfalseFormalin-Fixed and Paraffin-Embedded Samples for Next Generation Sequencing: Problems and Solutions.Over the years, increasing information has been asked of the pathologist: we have moved from a purely morphological diagnosis to biomolecular and genetic studies, which have made it possible to implement the use of molecular targeted therapies, such as anti-epidermal growth factor receptor (EGFR) molecules in EGFR-mutated lung cancer, for example. Today, next generation sequencing (NGS) has changed the approach to neoplasms, to the extent that, in a short time, it has gained a place of absolute importance and diagnostic, prognostic and therapeutic utility. In this scenario, formaldehyde-fixed and paraffin-embedded (FFPE) biological tissue samples are a source of clinical and molecular information. However, problems can arise in the genetic material (DNA and RNA) for use in NGS due to fixat2021-01-01T00:00:00Z2021 Sep2025-04-21T14:51:17.062Z2025-04-21T14:51:17.062ZS-EPMC85353263468086710.3390/genes12101472