{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rocha R"],"funding":["Fundação para a Ciência e a Tecnologia","Fundação para a Ciência e a Tecnologia","European Regional Development Fund"],"pagination":["2007"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8537266"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["9(10)"],"pubmed_abstract":["Serra da Estrela PDO cheese is the oldest traditional cheese manufactured in Portugal. In this work, its microbiome as well as the main raw materials used in cheese production, raw ewes' milk and thistle flowers (<i>Cynara cardunculus</i> L.), were characterized using next generation sequencing. Samples were accordingly retrieved from a local producer over two consecutive production campaigns and at different time periods within each campaign. The bacterial and fungi communities associated with each matrix were accessed through sequencing of V3-V4 and Internal Transcribed Spacer 2 regions of rRNA gene amplicons, respectively. A high microbial diversity was found associated to each matrix, differing significantly (<i>p</i> < 0.05) from each other. Over 500 taxa were identified in each analy"],"journal":["Microorganisms"],"pubmed_title":["Serra da Estrela PDO Cheese Microbiome as Revealed by Next Generation Sequencing."],"pmcid":["PMC8537266"],"funding_grant_id":["UIDP/05937/2020","UIDB/05937/2020","POCI–01–0247–FEDER–024524","POCI-01-0247-FEDER-024524"],"pubmed_authors":["Fernandes P","Santos J","Rocha R","Vaz Velho M"],"additional_accession":[]},"is_claimable":false,"name":"Serra da Estrela PDO Cheese Microbiome as Revealed by Next Generation Sequencing.","description":"Serra da Estrela PDO cheese is the oldest traditional cheese manufactured in Portugal. In this work, its microbiome as well as the main raw materials used in cheese production, raw ewes' milk and thistle flowers (<i>Cynara cardunculus</i> L.), were characterized using next generation sequencing. Samples were accordingly retrieved from a local producer over two consecutive production campaigns and at different time periods within each campaign. The bacterial and fungi communities associated with each matrix were accessed through sequencing of V3-V4 and Internal Transcribed Spacer 2 regions of rRNA gene amplicons, respectively. A high microbial diversity was found associated to each matrix, differing significantly (<i>p</i> < 0.05) from each other. Over 500 taxa were identified in each analy","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2026-05-03T03:10:17.15Z","creation":"2025-02-19T00:27:59.083Z"},"accession":"S-EPMC8537266","cross_references":{"pubmed":["34683326"],"doi":["10.3390/microorganisms9102007"]}}