biostudies-literatureUnknown21(20)Gonzalez-Gonzalez EThe COVID-19 pandemic has been the most critical public health issue in modern history due to its highly infectious and deathly potential, and the limited access to massive, low-cost, and reliable testing has significantly worsened the crisis. The recovery and the vaccination of millions of people against COVID-19 have made serological tests highly relevant to identify the presence and levels of SARS-CoV-2 antibodies. Due to its advantages, microfluidic-based technologies represent an attractive alternative to the conventional testing methodologies used for these purposes. In this work, we described the development of an automated ELISA on-chip capable of detecting anti-SARS-CoV-2 antibodies in serum samples from COVID-19 patients and vaccinated individuals. The colorimetric reactions were analyzed with a microplate reader. No statistically significant differences were observed when comparing the results of our automated ELISA on-chip against the ones obtained from a traditional ELISA on a microplate. Moreover, we demonstrated that it is possible to carry out the analysis of the colorimetric reaction by performing basic image analysis of photos taken with a smartphone, which constitutes a useful alternative when lacking specialized equipment or a laboratory setting. Our automated ELISA on-chip has the potential to be used in a clinical setting and mitigates some of the burden caused by testing deficiencies.Sensors (Basel, Switzerland)6785https://www.ebi.ac.uk/biostudies/studies/S-EPMC8539637biostudies-literatureAutomated ELISA On-Chip for the Detection of Anti-SARS-CoV-2 Antibodies.PMC8539637Gonzalez-Gonzalez ESoto-Garcia BYee-de Leon JRVelarde-Calvillo LPAraiz-Hernandez DAbarca-Blanco MFlores-Contreras EADelgado-Balderas JRYee-de Leon JFGarcia-Ramirez RDiaz-Armas GGAguilar-Avelar CAbarca-Blanco AEsparza MRodriguez-Sanchez IPfalseAutomated ELISA On-Chip for the Detection of Anti-SARS-CoV-2 Antibodies.The COVID-19 pandemic has been the most critical public health issue in modern history due to its highly infectious and deathly potential, and the limited access to massive, low-cost, and reliable testing has significantly worsened the crisis. The recovery and the vaccination of millions of people against COVID-19 have made serological tests highly relevant to identify the presence and levels of SARS-CoV-2 antibodies. Due to its advantages, microfluidic-based technologies represent an attractive alternative to the conventional testing methodologies used for these purposes. In this work, we described the development of an automated ELISA on-chip capable of detecting anti-SARS-CoV-2 antibodies in serum samples from COVID-19 patients and vaccinated individuals. The colorimetric reactions were analyzed with a microplate reader. No statistically significant differences were observed when comparing the results of our automated ELISA on-chip against the ones obtained from a traditional ELISA on a microplate. Moreover, we demonstrated that it is possible to carry out the analysis of the colorimetric reaction by performing basic image analysis of photos taken with a smartphone, which constitutes a useful alternative when lacking specialized equipment or a laboratory setting. Our automated ELISA on-chip has the potential to be used in a clinical setting and mitigates some of the burden caused by testing deficiencies.2021-01-01T00:00:00Z2021 Oct2026-06-15T03:11:36.491Z2026-06-15T03:08:20.082ZS-EPMC85396373469599810.3390/s21206785