biostudies-literatureRodrigues MQFundação para a Ciência e a TecnologiaEDUFLUVACTRANSVAC21621https://www.ebi.ac.uk/biostudies/studies/S-EPMC8540537biostudies-literatureUnknown13(10)In the last decade, the interest in ferritin-based vaccines has been increasing due to their safety and immunogenicity. Candidates against a wide range of pathogens are now on Phase I clinical trials namely for influenza, Epstein-Barr, and SARS-CoV-2 viruses. Manufacturing challenges related to particle heterogeneity, improper folding of fused antigens, and antigen interference with intersubunit interactions still need to be overcome. In addition, protocols need to be standardized so that the production bioprocess becomes reproducible, allowing ferritin-based therapeutics to become readily available. In this review, the building blocks that enable the formulation of ferritin-based vaccines at an experimental stage, including design, production, and purification are presented. Novel bioengiPharmaceuticsFunctionalizing Ferritin Nanoparticles for Vaccine Development.PMC8540537UIDB/04462/2020EXPL/BBB-BIO/1541/2013IF/01704/2014DFA/BD/8167/2020602640730964UIDP/04462/2020Rodrigues MQAlves PMRoldao AfalseFunctionalizing Ferritin Nanoparticles for Vaccine Development.In the last decade, the interest in ferritin-based vaccines has been increasing due to their safety and immunogenicity. Candidates against a wide range of pathogens are now on Phase I clinical trials namely for influenza, Epstein-Barr, and SARS-CoV-2 viruses. Manufacturing challenges related to particle heterogeneity, improper folding of fused antigens, and antigen interference with intersubunit interactions still need to be overcome. In addition, protocols need to be standardized so that the production bioprocess becomes reproducible, allowing ferritin-based therapeutics to become readily available. In this review, the building blocks that enable the formulation of ferritin-based vaccines at an experimental stage, including design, production, and purification are presented. Novel bioengi2021-01-01T00:00:00Z2021 Oct2025-04-05T12:56:38.776Z2025-04-05T12:56:38.776ZS-EPMC85405373468391410.3390/pharmaceutics13101621