biostudies-literatureBajgiran KRLouisiana Board of Regents1211https://www.ebi.ac.uk/biostudies/studies/S-EPMC8540845biostudies-literatureUnknown12(10)Droplet microfluidics offers a wide range of applications, including high-throughput drug screening and single-cell DNA amplification. However, these platforms are often limited to single-input conditions that prevent them from analyzing multiple input parameters (e.g., combined cellular treatments) in a single experiment. Droplet multiplexing will result in higher overall throughput, lowering cost of fabrication, and cutting down the hands-on time in number of applications such as single-cell analysis. Additionally, while lab-on-a-chip fabrication costs have decreased in recent years, the syringe pumps required for generating droplets of uniform shape and size remain cost-prohibitive for researchers interested in utilizing droplet microfluidics. This work investigates the potential of simMicromachinesSimultaneous Droplet Generation with In-Series Droplet T-Junctions Induced by Gravity-Induced Flow.PMC8540845LEQSF(2019-20)-RD-D-05Cordova ASElkhanoufi RDorman JABajgiran KRMelvin ATfalseSimultaneous Droplet Generation with In-Series Droplet T-Junctions Induced by Gravity-Induced Flow.Droplet microfluidics offers a wide range of applications, including high-throughput drug screening and single-cell DNA amplification. However, these platforms are often limited to single-input conditions that prevent them from analyzing multiple input parameters (e.g., combined cellular treatments) in a single experiment. Droplet multiplexing will result in higher overall throughput, lowering cost of fabrication, and cutting down the hands-on time in number of applications such as single-cell analysis. Additionally, while lab-on-a-chip fabrication costs have decreased in recent years, the syringe pumps required for generating droplets of uniform shape and size remain cost-prohibitive for researchers interested in utilizing droplet microfluidics. This work investigates the potential of sim2021-01-01T00:00:00Z2021 Oct2025-04-04T10:21:34.771Z2025-04-04T10:21:34.771ZS-EPMC85408453468326210.3390/mi12101211