biostudies-literatureMao SShanghai Science Committee FoundationNatural Science Foundation of China336https://www.ebi.ac.uk/biostudies/studies/S-EPMC8543852biostudies-literatureUnknown40(1)<h4>Background</h4>Circular RNAs (circRNAs) have been shown to play vital biological functions in various tumors, including prostate cancer (PCa). However, the roles of circRNAs in the metastasis of PCa remain unclear. In the present study, differentially expressed circRNAs associated with PCa metastasis were screened using high-throughput RNA sequencing, from which hsa_circ_0004296 was identified.<h4>Methods</h4>Quantitative real-time PCR (qRT-PCR) was used to detect the expression of circ_0004296 in PCa tissues and adjacent normal tissues as well as in blood and urine. Gain and loss of function experiments were performed to investigate the function of circ_0004296 in PCa. Bioinformatics analyses, RNA pull-down assay, and mass spectrometry were conducted to identify RNA-binding proteins. Journal of experimental & clinical cancer research : CRHsa_circ_0004296 inhibits metastasis of prostate cancer by interacting with EIF4A3 to prevent nuclear export of ETS1 mRNA.PMC854385219411967700814723898167254981802554Zheng ZMao SLi CGuo YZhang JMaskey NWang HYan YYao XYang FMa WZhang WWang RWu YfalseHsa_circ_0004296 inhibits metastasis of prostate cancer by interacting with EIF4A3 to prevent nuclear export of ETS1 mRNA.<h4>Background</h4>Circular RNAs (circRNAs) have been shown to play vital biological functions in various tumors, including prostate cancer (PCa). However, the roles of circRNAs in the metastasis of PCa remain unclear. In the present study, differentially expressed circRNAs associated with PCa metastasis were screened using high-throughput RNA sequencing, from which hsa_circ_0004296 was identified.<h4>Methods</h4>Quantitative real-time PCR (qRT-PCR) was used to detect the expression of circ_0004296 in PCa tissues and adjacent normal tissues as well as in blood and urine. Gain and loss of function experiments were performed to investigate the function of circ_0004296 in PCa. Bioinformatics analyses, RNA pull-down assay, and mass spectrometry were conducted to identify RNA-binding proteins. 2021-01-01T00:00:00Z2021 Oct2025-05-18T12:08:44.821Z2025-05-18T12:08:44.821ZS-EPMC85438523469678210.1186/s13046-021-02138-8