biostudies-literatureZhu JMOST | National Key Research and Development Program of ChinaDevelopment and Reform Commission of Shenzhen MunicipalityNational Natural Science Foundation of Chinae01119-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC8544893biostudies-literatureUnknown6(1)High-quality and comprehensive reference gene catalogs are essential for metagenomic research. The rather low diversity of samples used to construct existing catalogs of the mouse gut metagenome limits the numbers of identified genes in existing catalogs. We therefore established an expanded catalog of genes in the mouse gut metagenome (EMGC) containing >5.8 million genes by integrating 88 newly sequenced samples, 86 mouse gut-related bacterial genomes, and 3 existing gene catalogs. EMGC increases the number of nonredundant genes by more than 1 million genes compared to the so-far most extensive catalog. More than 60% of the genes in EMGC were assigned to <i>Bacteria</i>, with 54.20% being assigned to a phylum and 35.33% to a genus, while 30.39% were annotated at the KEGG orthology level. mSphereAn Expanded Gene Catalog of Mouse Gut Metagenomes.PMC854489381670606DRC-SZ [2015]1622018YFC1313800Ren HLi XZou YZhu JXiao LHan MMadsen LKristiansen KZhong HLi MfalseAn Expanded Gene Catalog of Mouse Gut Metagenomes.High-quality and comprehensive reference gene catalogs are essential for metagenomic research. The rather low diversity of samples used to construct existing catalogs of the mouse gut metagenome limits the numbers of identified genes in existing catalogs. We therefore established an expanded catalog of genes in the mouse gut metagenome (EMGC) containing >5.8 million genes by integrating 88 newly sequenced samples, 86 mouse gut-related bacterial genomes, and 3 existing gene catalogs. EMGC increases the number of nonredundant genes by more than 1 million genes compared to the so-far most extensive catalog. More than 60% of the genes in EMGC were assigned to <i>Bacteria</i>, with 54.20% being assigned to a phylum and 35.33% to a genus, while 30.39% were annotated at the KEGG orthology level. 2021-01-01T00:00:00Z2021 Feb2026-06-12T08:59:48.295Z2025-02-19T00:03:16.05ZS-EPMC85448933362751010.1128/mSphere.01119-20