biostudies-literatureHu POREFNCATS NIH HHSNCRR NIH HHSNIDDK NIH HHSNCI NIH HHSNIHNIAMS NIH HHS115906https://www.ebi.ac.uk/biostudies/studies/S-EPMC8546917biostudies-literatureUnknown147Type 1 diabetes (T1DM) impairs bone formation and fracture healing in humans. Akita mice carry a mutation in one allele of the insulin-2 (Ins2) gene, which leads to pancreatic beta cell dysfunction and hyperglycemia by 5-6 weeks age. We hypothesized that T1DM in Akita mice is associated with decreased bone mass, weaker bones, and impaired fracture healing. Ins2 ± (Akita) and wildtype (WT) males were subjected to femur fracture at 18-weeks age and healing assessed 3-21 days post-fracture. Non-fractured left femurs were assessed for morphology (microCT) and strength (bending or torsion) at 19-21 weeks age. Fractured right femurs were assessed for callus mechanics (torsion), morphology and composition (microCT and histology) and gene expression (qPCR). Both Akita and WT mice gained weight froBoneType 1 diabetic Akita mice have low bone mass and impaired fracture healing.PMC8546917P30 AR074992P30 CA091842R21 AR066798UL1 TR002345P30 AR057235P30 DK020579S10 RR023660R01 AR050211S10 RR027552Migotsky NGardner MJBuettmann EGMcKenzie JASilva MJHu PfalseType 1 diabetic Akita mice have low bone mass and impaired fracture healing.Type 1 diabetes (T1DM) impairs bone formation and fracture healing in humans. Akita mice carry a mutation in one allele of the insulin-2 (Ins2) gene, which leads to pancreatic beta cell dysfunction and hyperglycemia by 5-6 weeks age. We hypothesized that T1DM in Akita mice is associated with decreased bone mass, weaker bones, and impaired fracture healing. Ins2 ± (Akita) and wildtype (WT) males were subjected to femur fracture at 18-weeks age and healing assessed 3-21 days post-fracture. Non-fractured left femurs were assessed for morphology (microCT) and strength (bending or torsion) at 19-21 weeks age. Fractured right femurs were assessed for callus mechanics (torsion), morphology and composition (microCT and histology) and gene expression (qPCR). Both Akita and WT mice gained weight fro2021-01-01T00:00:00Z2021 Jun2026-05-05T03:13:15.997Z2025-04-19T18:17:55.096ZS-EPMC85469173366261110.1016/j.bone.2021.115906