biostudies-literatureUnknown10(1)Li DIntramuscular fat, as one of the most important palatability attribute of beef carcase, is the primary determinant of beef quality. The research of adipogenesis mechanism would provide new insight into intramuscular fatty deposition. Here, the role of microRNA-378 was investigated during bovine adipogenic differentiation. It was revealed that miR-378 expression exists variably in bovine major tissue and organs by RT-qPCR. It was predicted that miR-378 targets CaMKK2, as an AMPKα kinase, by DIANA Tools. For better research, primary preadipocytes with stable transfection for up-/down-regulated expression of miR-378 were constructed by lentiviral vectors with GFP gene. The analyses of qPCR showed that <i>PPARγ</i> and <i>adiponectin</i> mRNA levels increased, but <i>C/EBPβ, pref-1</i> and <i>Adipocyte483-492https://www.ebi.ac.uk/biostudies/studies/S-EPMC8547835biostudies-literatureMicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting <i>CaMKK2</i>.PMC8547835Liu HQu CLi YLi DZhang YZhang XWang HfalseMicroRNA-378 regulates adipogenic differentiation in bovine intramuscular preadipocytes by targeting <i>CaMKK2</i>.Intramuscular fat, as one of the most important palatability attribute of beef carcase, is the primary determinant of beef quality. The research of adipogenesis mechanism would provide new insight into intramuscular fatty deposition. Here, the role of microRNA-378 was investigated during bovine adipogenic differentiation. It was revealed that miR-378 expression exists variably in bovine major tissue and organs by RT-qPCR. It was predicted that miR-378 targets CaMKK2, as an AMPKα kinase, by DIANA Tools. For better research, primary preadipocytes with stable transfection for up-/down-regulated expression of miR-378 were constructed by lentiviral vectors with GFP gene. The analyses of qPCR showed that <i>PPARγ</i> and <i>adiponectin</i> mRNA levels increased, but <i>C/EBPβ, pref-1</i> and <i>2021-01-01T00:00:00Z2021 Dec2025-04-19T06:09:05.65Z2025-04-19T06:09:05.65ZS-EPMC85478353469386010.1080/21623945.2021.1982526