biostudies-literatureRomer TBKræftens BekæmpelseUniversity of Copenhagen | Det Sundhedsvidenskabelige Fakultet, Københavns UniversitetThe NEYE FoundationLundbeck Foundation1239-1250https://www.ebi.ac.uk/biostudies/studies/S-EPMC8548315biostudies-literatureUnknown125(9)<h4>Background</h4>Novel immunotherapies targeting cancer-associated truncated O-glycans Tn (GalNAcα-Ser/Thr) and STn (Neu5Acα2-6GalNacα-Ser/Thr) are promising strategies for cancer treatment. However, no comprehensive, antibody-based mapping of truncated O-glycans in tumours exist to guide drug development.<h4>Methods</h4>We used monoclonal antibodies to map the expression of truncated O-glycans in >700 tissue cores representing healthy and tumour tissues originating from breast, colon, lung, pancreas, skin, CNS and mesenchymal tissue. Patient-derived xenografts were used to evaluate Tn expression upon tumour engraftment.<h4>Results</h4>The Tn-antigen was highly expressed in breast (57%, n = 64), colorectal (51%, n = 140) and pancreatic (53%, n = 108) tumours, while STn was mainly observeBritish journal of cancerMapping of truncated O-glycans in cancers of epithelial and non-epithelial origin.PMC8548315313-2019-869Groen ASchnabel JWandall HHPedersen JWDabelsteen SRomer TBAasted MKMTan EHaue ADfalseMapping of truncated O-glycans in cancers of epithelial and non-epithelial origin.<h4>Background</h4>Novel immunotherapies targeting cancer-associated truncated O-glycans Tn (GalNAcα-Ser/Thr) and STn (Neu5Acα2-6GalNacα-Ser/Thr) are promising strategies for cancer treatment. However, no comprehensive, antibody-based mapping of truncated O-glycans in tumours exist to guide drug development.<h4>Methods</h4>We used monoclonal antibodies to map the expression of truncated O-glycans in >700 tissue cores representing healthy and tumour tissues originating from breast, colon, lung, pancreas, skin, CNS and mesenchymal tissue. Patient-derived xenografts were used to evaluate Tn expression upon tumour engraftment.<h4>Results</h4>The Tn-antigen was highly expressed in breast (57%, n = 64), colorectal (51%, n = 140) and pancreatic (53%, n = 108) tumours, while STn was mainly observe2021-01-01T00:00:00Z2021 Oct2025-04-22T05:38:37.109Z2025-04-05T21:26:25.784ZS-EPMC85483153452666610.1038/s41416-021-01530-7