{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Muthusamy S"],"funding":["Ministry of Science and Technology, Taiwan","Academia Sinica"],"pagination":["72"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8549234"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(1)"],"pubmed_abstract":["Background
During autophagy defense against invading microbes, certain lipid types are indispensable for generating specialized membrane-bound organelles. The lipid composition of autophagosomes remains obscure, as does the issue of how specific lipids and lipid-associated enzymes participate in autophagosome formation and maturation. Helicobacter pylori is auxotrophic for cholesterol and converts cholesterol to cholesteryl glucoside derivatives, including cholesteryl 6'-O-acyl-α-D-glucoside (CAG). We investigated how CAG and its biosynthetic acyltransferase assist H. pylori to escape host-cell autophagy.Methods
We applied a metabolite-tagging method to obtain fluorophore-containing cholesteryl glucosides that were utilized to understand their intracellular locations. H. py"],"journal":["Journal of biomedical science"],"pubmed_title":["Enhanced enzymatic production of cholesteryl 6'-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori."],"pmcid":["PMC8549234"],"funding_grant_id":["MOST-108-3114-Y-001-002","MOST-108-2113-M-001-001","AS-SUMMIT-109","AS-KPQ-109-BioMed"],"pubmed_authors":["Muthusamy S","Mondal S","Chen GC","Hsieh MY","Ko YA","Liu WC","Mong KT","Jan HM","Lin CH","Yang WY"],"additional_accession":[]},"is_claimable":false,"name":"Enhanced enzymatic production of cholesteryl 6'-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori.","description":"Background
During autophagy defense against invading microbes, certain lipid types are indispensable for generating specialized membrane-bound organelles. The lipid composition of autophagosomes remains obscure, as does the issue of how specific lipids and lipid-associated enzymes participate in autophagosome formation and maturation. Helicobacter pylori is auxotrophic for cholesterol and converts cholesterol to cholesteryl glucoside derivatives, including cholesteryl 6'-O-acyl-α-D-glucoside (CAG). We investigated how CAG and its biosynthetic acyltransferase assist H. pylori to escape host-cell autophagy.Methods
We applied a metabolite-tagging method to obtain fluorophore-containing cholesteryl glucosides that were utilized to understand their intracellular locations. H. py","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Oct","modification":"2025-04-19T15:36:02.854Z","creation":"2025-04-19T15:36:02.854Z"},"accession":"S-EPMC8549234","cross_references":{"pubmed":["34706729"],"doi":["10.1186/s12929-021-00768-w"]}}