biostudies-literatureMuthusamy SMinistry of Science and Technology, TaiwanAcademia Sinica72https://www.ebi.ac.uk/biostudies/studies/S-EPMC8549234biostudies-literatureUnknown28(1)<h4>Background</h4>During autophagy defense against invading microbes, certain lipid types are indispensable for generating specialized membrane-bound organelles. The lipid composition of autophagosomes remains obscure, as does the issue of how specific lipids and lipid-associated enzymes participate in autophagosome formation and maturation. Helicobacter pylori is auxotrophic for cholesterol and converts cholesterol to cholesteryl glucoside derivatives, including cholesteryl 6'-O-acyl-α-D-glucoside (CAG). We investigated how CAG and its biosynthetic acyltransferase assist H. pylori to escape host-cell autophagy.<h4>Methods</h4>We applied a metabolite-tagging method to obtain fluorophore-containing cholesteryl glucosides that were utilized to understand their intracellular locations. H. pyJournal of biomedical scienceEnhanced enzymatic production of cholesteryl 6'-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori.PMC8549234MOST-108-3114-Y-001-002MOST-108-2113-M-001-001AS-SUMMIT-109AS-KPQ-109-BioMedMuthusamy SMondal SChen GCHsieh MYKo YALiu WCMong KTJan HMLin CHYang WYfalseEnhanced enzymatic production of cholesteryl 6'-acylglucoside impairs lysosomal degradation for the intracellular survival of Helicobacter pylori.<h4>Background</h4>During autophagy defense against invading microbes, certain lipid types are indispensable for generating specialized membrane-bound organelles. The lipid composition of autophagosomes remains obscure, as does the issue of how specific lipids and lipid-associated enzymes participate in autophagosome formation and maturation. Helicobacter pylori is auxotrophic for cholesterol and converts cholesterol to cholesteryl glucoside derivatives, including cholesteryl 6'-O-acyl-α-D-glucoside (CAG). We investigated how CAG and its biosynthetic acyltransferase assist H. pylori to escape host-cell autophagy.<h4>Methods</h4>We applied a metabolite-tagging method to obtain fluorophore-containing cholesteryl glucosides that were utilized to understand their intracellular locations. H. py2021-01-01T00:00:00Z2021 Oct2025-04-19T15:36:02.854Z2025-04-19T15:36:02.854ZS-EPMC85492343470672910.1186/s12929-021-00768-w