{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Matsuzaki C"],"funding":["the Institute for Fermentation, Osaka","Japan Agency for Medical Research and Development","the Ministry of Health and Welfare of Japan and Public/Private R&amp;D Investment Strategic Expansion PrograM","Institute for Fermentation, Osaka"],"pagination":["1949097"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8550178"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["<i>Leuconostoc mesenteroides</i> strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination. Strain NTM048 includes three putative EPS-synthesizing genes, <i>gtf1</i> and <i>gtf2</i> for synthesizing glucose polymers, and <i>lvnS</i> for synthesizing fructose polymer. To elucidate the key polymer structure for adjuvanticity, two genes, <i>gtf1</i> and <i>gtf2</i>, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in <i>Escherichia coli</i>. Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan). Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS. Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS."],"journal":["Gut microbes"],"pubmed_title":["Enzymatically synthesized exopolysaccharide of a probiotic strain <i>Leuconostoc mesenteroides</i> NTM048 shows adjuvant activity to promote IgA antibody responses."],"pmcid":["PMC8550178"],"funding_grant_id":["G-2020-3-065","20gm1010007h0004","20ak0101068h0004"],"pubmed_authors":["Hisa K","Kunisawa J","Yamamoto K","Matsuzaki C","Higashimura Y","Tomabechi Y","Nakashima Y","Endo I","Itonori S","Hosomi K"],"additional_accession":[]},"is_claimable":false,"name":"Enzymatically synthesized exopolysaccharide of a probiotic strain <i>Leuconostoc mesenteroides</i> NTM048 shows adjuvant activity to promote IgA antibody responses.","description":"<i>Leuconostoc mesenteroides</i> strain NTM048 produces an exopolysaccharide (EPS; glucose polymers 94% and fructose polymers 6%) with adjuvanticity for mucosal vaccination. Strain NTM048 includes three putative EPS-synthesizing genes, <i>gtf1</i> and <i>gtf2</i> for synthesizing glucose polymers, and <i>lvnS</i> for synthesizing fructose polymer. To elucidate the key polymer structure for adjuvanticity, two genes, <i>gtf1</i> and <i>gtf2</i>, which were annotated as glycoside hydrolase family 70 enzyme genes, were expressed in <i>Escherichia coli</i>. Glycosyl-linkage composition analysis and NMR analysis showed that the recombinant enzyme Gtf1 produced a soluble form of α-1,6-glucan, whereas the recombinant enzyme Gtf2 produced glucans with approximately equal percentages of α-1,6- and α-1,3-glucose residues both in the supernatant (S-glucan) and as a precipitate (P-glucan). Comparison of polysaccharides synthesized by Gtf1, Gtf2, and LvnS revealed that Gtf2-S-glucan, which was produced in the supernatant by Gtf2 and formed particles of 7.8 µm, possessed 1.8-fold higher ability to stimulate IgA production from murine Peyer's patch cells than native NTM048 EPS. Evaluation of adjuvanticity by intranasal administration of mice with an antigen (ovalbumin) and Gtf2-S-glucan or NTM048 EPS showed that Gtf2-S-glucan induced the production of higher antigen-specific antibodies in the airway mucosa and plasma, suggesting a pivotal role of Gtf2-S-glucan in the adjuvanticity of NTM048 EPS.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Jan-Dec","modification":"2026-04-08T08:44:43.231Z","creation":"2025-02-19T01:25:51.954Z"},"accession":"S-EPMC8550178","cross_references":{"pubmed":["34288820"],"doi":["10.1080/19490976.2021.1949097"]}}