biostudies-literatureMcKay Fletcher DMEuropean Research CouncilNatural Environment Research CouncilBiotechnology and Biological Sciences Research CouncilEngineering and Physical Sciences Research Council69-89https://www.ebi.ac.uk/biostudies/studies/S-EPMC8550755biostudies-literatureUnknown461(1-2)<h4>Aims</h4>Organic acid exudation by plant roots is thought to promote phosphate (P) solubilisation and bioavailability in soils with poorly available nutrients. Here we describe a new combined experimental (microdialysis) and modelling approach to quantify citrate-enhanced P desorption and its importance for root P uptake.<h4>Methods</h4>To mimic the rhizosphere, microdialysis probes were placed in soil and perfused with citrate solutions (0.1, 1.0 and 10 mM) and the amount of P recovered from soil used to quantify rhizosphere P availability. Parameters in a mathematical model describing probe P uptake, citrate exudation, P movement and citrate-enhanced desorption were fit to the experimental data. These parameters were used in a model of a root which exuded citrate and absorbed P. The Plant and soilQuantifying citrate-enhanced phosphate root uptake using microdialysis.PMC8550755BB/L025620/1BB/L025620/1, BB/P004180/16468091636114BB/P004180/1EP/M020355/1NE/L00237/1NE/K01093X/1BB/I024283/1Jones DLRoose TShaw Rvan Veelen AMcKay Fletcher DMSanchez-Rodriguez ARDaly KRfalseQuantifying citrate-enhanced phosphate root uptake using microdialysis.<h4>Aims</h4>Organic acid exudation by plant roots is thought to promote phosphate (P) solubilisation and bioavailability in soils with poorly available nutrients. Here we describe a new combined experimental (microdialysis) and modelling approach to quantify citrate-enhanced P desorption and its importance for root P uptake.<h4>Methods</h4>To mimic the rhizosphere, microdialysis probes were placed in soil and perfused with citrate solutions (0.1, 1.0 and 10 mM) and the amount of P recovered from soil used to quantify rhizosphere P availability. Parameters in a mathematical model describing probe P uptake, citrate exudation, P movement and citrate-enhanced desorption were fit to the experimental data. These parameters were used in a model of a root which exuded citrate and absorbed P. The 2021-01-01T00:00:00Z20212026-05-08T17:13:17.207Z2022-02-11T12:26:00.538ZS-EPMC85507553472020710.1007/s11104-019-04376-4