biostudies-literatureUnknown12Zaveska Drabkova LGrantová Agentura České RepublikyMinisterstvo Å kolství Mládeže a TělovýchovyPlant microgametogenesis involves stages leading to the progressive development of unicellular microspores into mature pollen. Despite the active and continuing interest in the study of male reproductive development, little is still known about the hormonomics at each ontogenetic stage. In this work, we characterized the profiles and dynamics of phytohormones during the process of microgametogenesis in four <i>Nicotiana</i> species (<i>Nicotiana tabacum</i>, <i>Nicotiana alata</i>, <i>Nicotiana langsdorffii</i>, and <i>Nicotiana mutabilis</i>). Taking advantage of advanced HPLC-ESI-MS/MS, twenty to thirty endogenous hormone derivatives were identified throughout pollen ontogenesis, including cytokinins, auxins, ABA and its derivatives, jasmonates, and phenolic compounds. The spectra of endFrontiers in plant science735451https://www.ebi.ac.uk/biostudies/studies/S-EPMC8553967biostudies-literatureHormonome Dynamics During Microgametogenesis in Different <i>Nicotiana</i> Species.PMC8553967Pokorna EHonys DKurkova JMotyka VSteinbachova LDobrev PIZaveska Drabkova LfalseHormonome Dynamics During Microgametogenesis in Different <i>Nicotiana</i> Species.Plant microgametogenesis involves stages leading to the progressive development of unicellular microspores into mature pollen. Despite the active and continuing interest in the study of male reproductive development, little is still known about the hormonomics at each ontogenetic stage. In this work, we characterized the profiles and dynamics of phytohormones during the process of microgametogenesis in four <i>Nicotiana</i> species (<i>Nicotiana tabacum</i>, <i>Nicotiana alata</i>, <i>Nicotiana langsdorffii</i>, and <i>Nicotiana mutabilis</i>). Taking advantage of advanced HPLC-ESI-MS/MS, twenty to thirty endogenous hormone derivatives were identified throughout pollen ontogenesis, including cytokinins, auxins, ABA and its derivatives, jasmonates, and phenolic compounds. The spectra of end2021-01-01T00:00:00Z20212025-04-19T08:05:13.442Z2025-04-19T08:05:13.442ZS-EPMC85539673472146410.3389/fpls.2021.735451