{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12"],"submitter":["Niu M"],"pubmed_abstract":["Animals, from insects to humans, exhibit obvious diurnal rhythmicity of feeding behavior. Serving as a genetic animal model, <i>Drosophila</i> has been reported to display feeding rhythms; however, related investigations are limited due to the lack of suitable and practical methods. Here, we present a video recording-based analytical method, namely, <i>Drosophila</i> Feeding Rhythm Analysis Method (dFRAME). Using our newly developed computer program, FlyFeeding, we extracted the movement track of individual flies and characterized their food-approaching behavior. To distinguish feeding and no-feeding events, we utilized high-magnification video recording to optimize our method by setting cut-off thresholds to eliminate the interference of no-feeding events. Furthermore, we verified that this method is applicable to both female and male flies and for all periods of the day. Using this method, we analyzed long-term feeding status of wild-type and <i>period</i> mutant flies. The results recaptured previously reported feeding rhythms and revealed detailed profiles of feeding patterns in these flies under either light/dark cycles or constant dark environments. Together, our dFRAME method enables a long-term, stable, reliable, and subtle analysis of feeding behavior in <i>Drosophila</i>. High-throughput studies in this powerful genetic animal model will gain great insights into the molecular and neural mechanisms of feeding rhythms."],"journal":["Frontiers in genetics"],"pagination":["763200"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8554052"],"repository":["biostudies-literature"],"pubmed_title":["dFRAME: A Video Recording-Based Analytical Method for Studying Feeding Rhythm in <i>Drosophila</i>."],"pmcid":["PMC8554052"],"pubmed_authors":["Li W","Li Y","Zhang X","Niu M","Wang J"],"additional_accession":[]},"is_claimable":false,"name":"dFRAME: A Video Recording-Based Analytical Method for Studying Feeding Rhythm in <i>Drosophila</i>.","description":"Animals, from insects to humans, exhibit obvious diurnal rhythmicity of feeding behavior. Serving as a genetic animal model, <i>Drosophila</i> has been reported to display feeding rhythms; however, related investigations are limited due to the lack of suitable and practical methods. Here, we present a video recording-based analytical method, namely, <i>Drosophila</i> Feeding Rhythm Analysis Method (dFRAME). Using our newly developed computer program, FlyFeeding, we extracted the movement track of individual flies and characterized their food-approaching behavior. To distinguish feeding and no-feeding events, we utilized high-magnification video recording to optimize our method by setting cut-off thresholds to eliminate the interference of no-feeding events. Furthermore, we verified that this method is applicable to both female and male flies and for all periods of the day. Using this method, we analyzed long-term feeding status of wild-type and <i>period</i> mutant flies. The results recaptured previously reported feeding rhythms and revealed detailed profiles of feeding patterns in these flies under either light/dark cycles or constant dark environments. Together, our dFRAME method enables a long-term, stable, reliable, and subtle analysis of feeding behavior in <i>Drosophila</i>. High-throughput studies in this powerful genetic animal model will gain great insights into the molecular and neural mechanisms of feeding rhythms.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021","modification":"2025-04-18T19:12:00.5Z","creation":"2022-02-11T12:19:16.48Z"},"accession":"S-EPMC8554052","cross_references":{"pubmed":["34721548"],"doi":["10.3389/fgene.2021.763200"]}}