biostudies-literatureUnknown16(10)Touati AIn a previous study, we developed a Multi-Locus VNTRs Analysis (MLVA) typing system, called MLVA-5, for the discrimination of Chlamydia trachomatis genovar E strain. The results suggested the clonal spread of a MLVA-5 type 21 strain among men who have sex with men (MSM). We applied the MLVA-5 typing method on 157 French anorectal genovar E specimens and 19 Swedish specimens collected between 2010 and 2015. A total of 29 MLVA-5 types was obtained, with three predominant types among French samples: 78 specimens belonged to MLVA-5 type 21, two other types, 11 and 13, included 9 and 14 specimens, respectively. In 15 cases, one unique MLVA-5 type was observed for a single patient, 7 of which were new types not previously described. The distribution of MLVA-5 types according to sexual orientatioPloS onee0259274https://www.ebi.ac.uk/biostudies/studies/S-EPMC8555804biostudies-literatureSpread of clonal genovar E Chlamydia trachomatis among men who have sex with men.PMC8555804Bebear CLaurier-Nadalie CTouati Ade Barbeyrac BPeuchant OHerrmann BHenin NfalseSpread of clonal genovar E Chlamydia trachomatis among men who have sex with men.In a previous study, we developed a Multi-Locus VNTRs Analysis (MLVA) typing system, called MLVA-5, for the discrimination of Chlamydia trachomatis genovar E strain. The results suggested the clonal spread of a MLVA-5 type 21 strain among men who have sex with men (MSM). We applied the MLVA-5 typing method on 157 French anorectal genovar E specimens and 19 Swedish specimens collected between 2010 and 2015. A total of 29 MLVA-5 types was obtained, with three predominant types among French samples: 78 specimens belonged to MLVA-5 type 21, two other types, 11 and 13, included 9 and 14 specimens, respectively. In 15 cases, one unique MLVA-5 type was observed for a single patient, 7 of which were new types not previously described. The distribution of MLVA-5 types according to sexual orientatio2021-01-01T00:00:00Z20212025-04-04T14:08:35.691Z2025-04-04T14:08:35.691ZS-EPMC85558043471486510.1371/journal.pone.0259274