{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Payne NC"],"funding":["NIAID NIH HHS","Karlsruher Institut für Technologie","U.S. Department of Health & Human Services | National Institutes of Health","National Science Foundation"],"pagination":["1168-1177"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8555866"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(11)"],"pubmed_abstract":["The pace of progress in biomedical research directly depends on techniques that enable the quantitative interrogation of interactions between proteins and other biopolymers, or with their small-molecule ligands. Time-resolved Förster resonance energy transfer (TR-FRET) assay platforms offer high sensitivity and specificity. However, the paucity of accessible and biocompatible luminescent lanthanide complexes, which are essential reagents for TR-FRET-based approaches, and their poor cellular permeability have limited broader adaptation of TR-FRET beyond homogeneous and extracellular assay applications. Here, we report the development of CoraFluors, a new class of macrotricyclic terbium complexes, which are synthetically readily accessible, stable in biological media and exhibit photophysica"],"journal":["Nature chemical biology"],"pubmed_title":["Bright and stable luminescent probes for target engagement profiling in live cells."],"pmcid":["PMC8555866"],"funding_grant_id":["DGE1745303","NSF 1830941","R01AI143723","R01 AI143723","R21AI13298"],"pubmed_authors":["Singh K","Payne NC","Kalyakina AS","Tye MA","Mazitschek R"],"additional_accession":[]},"is_claimable":false,"name":"Bright and stable luminescent probes for target engagement profiling in live cells.","description":"The pace of progress in biomedical research directly depends on techniques that enable the quantitative interrogation of interactions between proteins and other biopolymers, or with their small-molecule ligands. Time-resolved Förster resonance energy transfer (TR-FRET) assay platforms offer high sensitivity and specificity. However, the paucity of accessible and biocompatible luminescent lanthanide complexes, which are essential reagents for TR-FRET-based approaches, and their poor cellular permeability have limited broader adaptation of TR-FRET beyond homogeneous and extracellular assay applications. Here, we report the development of CoraFluors, a new class of macrotricyclic terbium complexes, which are synthetically readily accessible, stable in biological media and exhibit photophysica","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2026-05-31T06:04:53.075Z","creation":"2025-02-19T01:55:34.627Z"},"accession":"S-EPMC8555866","cross_references":{"pubmed":["34675420"],"doi":["10.1038/s41589-021-00877-5"]}}