biostudies-literaturePayne NCNIAID NIH HHSKarlsruher Institut für TechnologieU.S. Department of Health & Human Services | National Institutes of HealthNational Science Foundation1168-1177https://www.ebi.ac.uk/biostudies/studies/S-EPMC8555866biostudies-literatureUnknown17(11)The pace of progress in biomedical research directly depends on techniques that enable the quantitative interrogation of interactions between proteins and other biopolymers, or with their small-molecule ligands. Time-resolved Förster resonance energy transfer (TR-FRET) assay platforms offer high sensitivity and specificity. However, the paucity of accessible and biocompatible luminescent lanthanide complexes, which are essential reagents for TR-FRET-based approaches, and their poor cellular permeability have limited broader adaptation of TR-FRET beyond homogeneous and extracellular assay applications. Here, we report the development of CoraFluors, a new class of macrotricyclic terbium complexes, which are synthetically readily accessible, stable in biological media and exhibit photophysicaNature chemical biologyBright and stable luminescent probes for target engagement profiling in live cells.PMC8555866DGE1745303NSF 1830941R01AI143723R01 AI143723R21AI13298Singh KPayne NCKalyakina ASTye MAMazitschek RfalseBright and stable luminescent probes for target engagement profiling in live cells.The pace of progress in biomedical research directly depends on techniques that enable the quantitative interrogation of interactions between proteins and other biopolymers, or with their small-molecule ligands. Time-resolved Förster resonance energy transfer (TR-FRET) assay platforms offer high sensitivity and specificity. However, the paucity of accessible and biocompatible luminescent lanthanide complexes, which are essential reagents for TR-FRET-based approaches, and their poor cellular permeability have limited broader adaptation of TR-FRET beyond homogeneous and extracellular assay applications. Here, we report the development of CoraFluors, a new class of macrotricyclic terbium complexes, which are synthetically readily accessible, stable in biological media and exhibit photophysica2021-01-01T00:00:00Z2021 Nov2026-05-31T06:04:53.075Z2025-02-19T01:55:34.627ZS-EPMC85558663467542010.1038/s41589-021-00877-5