biostudies-literatureCorvigno SAmerican Cancer SocietyNCI NIH HHSFrank McGraw Memorial Chair in Cancer Researchthe MD Anderson Ovarian Cancer SPORENational Institutes of HealthNIH HHS3545-3555https://www.ebi.ac.uk/biostudies/studies/S-EPMC8557133biostudies-literatureUnknown147(12)<h4>Purpose</h4>An in-depth analysis of the tumor microenvironment of ovarian cancer is needed. The purpose of this study was to elucidate the architecture of the immune microenvironment of high-grade serous ovarian cancers (HGSCs) with or without BRCA1 and BRCA2 mutations.<h4>Methods</h4>A cohort of highly annotated HGSC patients with known germline BRCA1 and BRCA2 status was selected, and pretreatment tumor tissue specimens were analyzed with a multiplexed staining technique aimed at detecting lymphocytes, macrophages, and fibroblasts in the whole tumor area and in specific regions including epithelium, stroma, and perivascular areas.<h4>Results</h4>BRCA1- or BRCA2-mutated tumors showed a more immunogenic microenvironment, characterized by a higher abundance of CD8⁺ and PD-L1<Journal of cancer research and clinical oncologyImmune microenvironment composition in high-grade serous ovarian cancers based on BRCA mutational status.PMC8557133R35 CA209904R50 CA243707P30 CA016672U01 CA213759P50 CA217685,R35 CA209904, U01 CA213759R01 CA247220P50 CA217685Sood AKLiu JHu WWestin SNCorvigno SJennings NBFleming NDFellman BZhong YBurks JKfalseImmune microenvironment composition in high-grade serous ovarian cancers based on BRCA mutational status.<h4>Purpose</h4>An in-depth analysis of the tumor microenvironment of ovarian cancer is needed. The purpose of this study was to elucidate the architecture of the immune microenvironment of high-grade serous ovarian cancers (HGSCs) with or without BRCA1 and BRCA2 mutations.<h4>Methods</h4>A cohort of highly annotated HGSC patients with known germline BRCA1 and BRCA2 status was selected, and pretreatment tumor tissue specimens were analyzed with a multiplexed staining technique aimed at detecting lymphocytes, macrophages, and fibroblasts in the whole tumor area and in specific regions including epithelium, stroma, and perivascular areas.<h4>Results</h4>BRCA1- or BRCA2-mutated tumors showed a more immunogenic microenvironment, characterized by a higher abundance of CD8⁺ and PD-L1<2021-01-01T00:00:00Z2021 Dec2025-04-05T09:43:49.297Z2025-04-05T09:43:49.297ZS-EPMC85571333447657610.1007/s00432-021-03778-1