biostudies-literatureUnknown38(3)Sako KAbiotic stresses, such as high light and salinity, are major factors that limit crop productivity and sustainability worldwide. Chemical priming is a promising strategy for improving the abiotic stress tolerance of plants. Recently, we discovered that ethanol enhances high-salinity stress tolerance in <i>Arabidopsis thaliana</i> and rice by detoxifying reactive oxygen species (ROS). However, the effect of ethanol on other abiotic stress responses is unclear. Therefore, we investigated the effect of ethanol on the high-light stress response. Measurement of chlorophyll fluorescence showed that ethanol mitigates photoinhibition under high-light stress. Staining with 3,3'-diaminobenzidine (DAB) showed that the accumulation of hydrogen peroxide (H₂O₂) was inhibited by ethanol under high-light stress conditions in <i>A. thaliana</i>. We found that ethanol increased the gene expressions and enzymatic activities of antioxidative enzymes, including <i>ASCORBATE PEROXIDASE1</i> (<i>AtAPX1</i>), <i>Catalase (AtCAT1 and AtCAT2)</i>. Moreover, the expression of flavonoid biosynthetic genes and anthocyanin contents were upregulated by ethanol treatment during exposure to high-light stress. These results imply that ethanol alleviates oxidative damage from high-light stress in <i>A. thaliana</i> by suppressing ROS accumulation. Our findings support the hypothesis that ethanol improves tolerance to multiple stresses in field-grown crops.Plant biotechnology (Tokyo, Japan)339-344https://www.ebi.ac.uk/biostudies/studies/S-EPMC8562572biostudies-literatureExogenous ethanol treatment alleviates oxidative damage of <i>Arabidopsis thaliana</i> under conditions of high-light stress.PMC8562572Nagashima RTamoi MSako KSeki MfalseExogenous ethanol treatment alleviates oxidative damage of <i>Arabidopsis thaliana</i> under conditions of high-light stress.Abiotic stresses, such as high light and salinity, are major factors that limit crop productivity and sustainability worldwide. Chemical priming is a promising strategy for improving the abiotic stress tolerance of plants. Recently, we discovered that ethanol enhances high-salinity stress tolerance in <i>Arabidopsis thaliana</i> and rice by detoxifying reactive oxygen species (ROS). However, the effect of ethanol on other abiotic stress responses is unclear. Therefore, we investigated the effect of ethanol on the high-light stress response. Measurement of chlorophyll fluorescence showed that ethanol mitigates photoinhibition under high-light stress. Staining with 3,3'-diaminobenzidine (DAB) showed that the accumulation of hydrogen peroxide (H₂O₂) was inhibited by ethanol under high-light stress conditions in <i>A. thaliana</i>. We found that ethanol increased the gene expressions and enzymatic activities of antioxidative enzymes, including <i>ASCORBATE PEROXIDASE1</i> (<i>AtAPX1</i>), <i>Catalase (AtCAT1 and AtCAT2)</i>. Moreover, the expression of flavonoid biosynthetic genes and anthocyanin contents were upregulated by ethanol treatment during exposure to high-light stress. These results imply that ethanol alleviates oxidative damage from high-light stress in <i>A. thaliana</i> by suppressing ROS accumulation. Our findings support the hypothesis that ethanol improves tolerance to multiple stresses in field-grown crops.2021-01-01T00:00:00Z2021 Sep2024-02-15T15:02:25.489Z2022-02-11T12:51:55.639ZS-EPMC85625723478282110.5511/plantbiotechnology.21.0715a