biostudies-literatureLee JJNCI NIH HHSNIHCancer Prevention and Research Institute of Texas5912-5921https://www.ebi.ac.uk/biostudies/studies/S-EPMC8563410biostudies-literatureUnknown27(21)<h4>Purpose</h4>Precision medicine approaches in pancreatic ductal adenocarcinoma (PDAC) are imperative for improving disease outcomes. With molecular subtypes of PDAC gaining relevance in the context of therapeutic stratification, the ability to characterize heterogeneity of cancer-specific gene expression patterns is of great interest. In addition, understanding patterns of immune evasion within PDAC is of importance as novel immunotherapeutic strategies are developed.<h4>Experimental design</h4>Single-cell RNA sequencing (scRNA-seq) is readily applicable to limited biopsies from human primary and metastatic PDAC and identifies most cancers as being an admixture of previously described epithelial transcriptomic subtypes.<h4>Results</h4>Integrative analyses of our data provide an in-depthClinical cancer research : an official journal of the American Association for Cancer ResearchElucidation of Tumor-Stromal Heterogeneity and the Ligand-Receptor Interactome by Single-Cell Transcriptomics in Real-world Pancreatic Cancer Biopsies.PMC8563410U01CA200468U54CA096297U54 CA096300U01 CA196403CPRIT RR140012U01 CA200468T32 CA217789U54CA096300T32 CA009599R01CA227518-01A1U01CA196403P50CA221707T32CA009599R01 CA227517SE-2616/2-1U54 CA096297P50 CA221707T32CA217789Taniguchi CMMonberg MEHuang JWeston BRBernard VStephens BMBernatchez CSemaan AHaymaker CLRajapakshe KIBhutani MSMaitra ALin DLee JJGuerrero PAfalseElucidation of Tumor-Stromal Heterogeneity and the Ligand-Receptor Interactome by Single-Cell Transcriptomics in Real-world Pancreatic Cancer Biopsies.<h4>Purpose</h4>Precision medicine approaches in pancreatic ductal adenocarcinoma (PDAC) are imperative for improving disease outcomes. With molecular subtypes of PDAC gaining relevance in the context of therapeutic stratification, the ability to characterize heterogeneity of cancer-specific gene expression patterns is of great interest. In addition, understanding patterns of immune evasion within PDAC is of importance as novel immunotherapeutic strategies are developed.<h4>Experimental design</h4>Single-cell RNA sequencing (scRNA-seq) is readily applicable to limited biopsies from human primary and metastatic PDAC and identifies most cancers as being an admixture of previously described epithelial transcriptomic subtypes.<h4>Results</h4>Integrative analyses of our data provide an in-depth2021-01-01T00:00:00Z2021 Nov2026-05-31T06:06:00.747Z2025-02-19T01:55:22.406ZS-EPMC85634103442643910.1158/1078-0432.ccr-20-392510.1158/1078-0432.CCR-20-3925