{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Calvete JJ"],"funding":["Ministerio de Ciencia e Innovaci??n","Technische Universit??t Berlin","Agilent Technologies","European Regional Development Fund"],"pagination":["5064-5078"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8576837"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(11)"],"pubmed_abstract":["We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus <i>Walterinnesia</i>, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>. The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for protein identification and a parallel reverse-phase microbore high-performance liquid chromatograph (RP-μHPLC) on-line to inductively coupled plasma (ICP-MS/MS) elemental mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QToF MS). This allows to continuou"],"journal":["Journal of proteome research"],"pubmed_title":["Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>."],"pmcid":["PMC8576837"],"funding_grant_id":["BFU2017-89103-P","PGC2018-098290-B-I00","PID2019-109698GB-I00"],"pubmed_authors":["Hempel BF","Carranza S","Sussmuth RD","Els J","Heiss P","Encinar JR","Pla D","Petras D","Nalbantsoy A","Damm M","Gocmen B","Calvete JJ","John EBO","Nosti AJ","Calderon-Celis F"],"additional_accession":[]},"is_claimable":false,"name":"Combined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>.","description":"We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus <i>Walterinnesia</i>, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>. The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for protein identification and a parallel reverse-phase microbore high-performance liquid chromatograph (RP-μHPLC) on-line to inductively coupled plasma (ICP-MS/MS) elemental mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QToF MS). This allows to continuou","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2026-04-08T12:10:38.346Z","creation":"2022-02-11T12:44:07.758Z"},"accession":"S-EPMC8576837","cross_references":{"pubmed":["34606723"],"doi":["10.1021/acs.jproteome.1c00608"]}}