biostudies-literatureCalvete JJMinisterio de Ciencia e Innovaci??nTechnische Universit??t BerlinAgilent TechnologiesEuropean Regional Development Fund5064-5078https://www.ebi.ac.uk/biostudies/studies/S-EPMC8576837biostudies-literatureUnknown20(11)We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus <i>Walterinnesia</i>, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>. The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for protein identification and a parallel reverse-phase microbore high-performance liquid chromatograph (RP-μHPLC) on-line to inductively coupled plasma (ICP-MS/MS) elemental mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QToF MS). This allows to continuouJournal of proteome researchCombined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>.PMC8576837BFU2017-89103-PPGC2018-098290-B-I00PID2019-109698GB-I00Hempel BFCarranza SSussmuth RDEls JHeiss PEncinar JRPla DPetras DNalbantsoy ADamm MGocmen BCalvete JJJohn EBONosti AJCalderon-Celis FfalseCombined Molecular and Elemental Mass Spectrometry Approaches for Absolute Quantification of Proteomes: Application to the Venomics Characterization of the Two Species of Desert Black Cobras, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>.We report a novel hybrid, molecular and elemental mass spectrometry (MS) setup for the absolute quantification of snake venom proteomes shown here for two desert black cobra species within the genus <i>Walterinnesia</i>, <i>Walterinnesia aegyptia</i> and <i>Walterinnesia morgani</i>. The experimental design includes the decomplexation of the venom samples by reverse-phase chromatography independently coupled to four mass spectrometry systems: the combined bottom-up and top-down molecular MS for protein identification and a parallel reverse-phase microbore high-performance liquid chromatograph (RP-μHPLC) on-line to inductively coupled plasma (ICP-MS/MS) elemental mass spectrometry and electrospray ionization quadrupole time-of-flight mass spectrometry (ESI-QToF MS). This allows to continuou2021-01-01T00:00:00Z2021 Nov2026-04-08T12:10:38.346Z2022-02-11T12:44:07.758ZS-EPMC85768373460672310.1021/acs.jproteome.1c00608