{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhou W"],"funding":["National Institute of Allergy and Infectious Diseases","Thailand Science Research and Innovation","NIAID NIH HHS","National Natural Science Foundation of China","NIH HHS"],"pagination":["2411-2419"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8577183"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["84(9)"],"pubmed_abstract":["The soil bacterium <i>Streptomyces pactum</i> ATCC 27456 produces a number of polyketide natural products. Among them is NFAT-133, an inhibitor of the nuclear factor of activated T cells (NFAT) that suppresses interleukin-2 (IL-2) expression and T cell proliferation. Biosynthetic gene inactivation in the ATCC 27456 strain revealed the ability of this strain to produce other polyketide compounds including analogues of NFAT-133. Consequently, seven new derivatives of NFAT-133, TM-129-TM-135, together with a known compound, panowamycin A, were isolated from the culture broth of <i>S. pactum</i> ATCC 27456 Δ<i>ptmTDQ</i>. Their chemical structures were elucidated on the basis of their HRESIMS, 1D and 2D NMR spectroscopy, and ECD calculation and spectral data. NFAT-133, TM-132, TM-135, and panowamycin A showed no antibacterial activity or cytotoxicity, but weakly reduced the production of LPS-induced nitric oxide in RAW264.7 cells in a dose-dependent manner. A revised chemical structure of panowamycin A and proposed modes of formation of the new NFAT-133 analogues are also presented."],"journal":["Journal of natural products"],"pubmed_title":["Identification and Biological Activity of NFAT-133 Congeners from <i>Streptomyces pactum</i>."],"pmcid":["PMC8577183"],"funding_grant_id":["S10 OD018518","AI129957","81872795","R01 AI129957","PHD/0007/2558"],"pubmed_authors":["Lan WJ","Ju Z","Liu XJ","Mahmud T","Zhou W","Posri P"],"additional_accession":[]},"is_claimable":false,"name":"Identification and Biological Activity of NFAT-133 Congeners from <i>Streptomyces pactum</i>.","description":"The soil bacterium <i>Streptomyces pactum</i> ATCC 27456 produces a number of polyketide natural products. Among them is NFAT-133, an inhibitor of the nuclear factor of activated T cells (NFAT) that suppresses interleukin-2 (IL-2) expression and T cell proliferation. Biosynthetic gene inactivation in the ATCC 27456 strain revealed the ability of this strain to produce other polyketide compounds including analogues of NFAT-133. Consequently, seven new derivatives of NFAT-133, TM-129-TM-135, together with a known compound, panowamycin A, were isolated from the culture broth of <i>S. pactum</i> ATCC 27456 Δ<i>ptmTDQ</i>. Their chemical structures were elucidated on the basis of their HRESIMS, 1D and 2D NMR spectroscopy, and ECD calculation and spectral data. NFAT-133, TM-132, TM-135, and panowamycin A showed no antibacterial activity or cytotoxicity, but weakly reduced the production of LPS-induced nitric oxide in RAW264.7 cells in a dose-dependent manner. A revised chemical structure of panowamycin A and proposed modes of formation of the new NFAT-133 analogues are also presented.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Sep","modification":"2025-04-04T13:57:39.762Z","creation":"2025-04-04T13:57:39.762Z"},"accession":"S-EPMC8577183","cross_references":{"pubmed":["34519213"],"doi":["10.1021/acs.jnatprod.1c00152"]}}