{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lihanova Y"],"funding":["Deutsche Forschungsgemeinschaft","Peter und Traudl Engelhorn Stiftung","Fond der Chemischen Industrie e. V"],"pagination":["1858-1866"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC8583172"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["18(11)"],"pubmed_abstract":["Nine distinct classes of self-cleaving ribozymes are known to date, of which the pistol ribozyme class was discovered only 5 years ago. Self-cleaving ribozymes are able to cleave their own phosphodiester backbone at a specific site with rates much higher than those of spontaneous RNA degradation. Our study focuses on a bioinformatically predicted pistol ribozyme from the bacterium <i>Paenibacillus polymyxa</i>. We provide a biochemical characterization of this ribozyme, which includes an investigation of the effect of various metal ions on ribozyme cleavage and a kinetic analysis of ribozyme activity under increasing Mg<sup>2+</sup> concentrations and pH. Based on the obtained results, we discuss a possible catalytic role of divalent metal ions. Moreover, we investigated the ligation activ"],"journal":["RNA biology"],"pubmed_title":["Biochemical analysis of cleavage and ligation activities of the pistol ribozyme from <i>Paenibacillus polymyxa</i>."],"pmcid":["PMC8583172"],"funding_grant_id":["LU1889/4-1"],"pubmed_authors":["Weinberg CE","Lihanova Y"],"additional_accession":[]},"is_claimable":false,"name":"Biochemical analysis of cleavage and ligation activities of the pistol ribozyme from <i>Paenibacillus polymyxa</i>.","description":"Nine distinct classes of self-cleaving ribozymes are known to date, of which the pistol ribozyme class was discovered only 5 years ago. Self-cleaving ribozymes are able to cleave their own phosphodiester backbone at a specific site with rates much higher than those of spontaneous RNA degradation. Our study focuses on a bioinformatically predicted pistol ribozyme from the bacterium <i>Paenibacillus polymyxa</i>. We provide a biochemical characterization of this ribozyme, which includes an investigation of the effect of various metal ions on ribozyme cleavage and a kinetic analysis of ribozyme activity under increasing Mg<sup>2+</sup> concentrations and pH. Based on the obtained results, we discuss a possible catalytic role of divalent metal ions. Moreover, we investigated the ligation activ","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Nov","modification":"2026-06-18T08:52:03.069Z","creation":"2025-04-05T14:05:35.335Z"},"accession":"S-EPMC8583172","cross_references":{"pubmed":["33622172"],"doi":["10.1080/15476286.2021.1874706"]}}